Role of p38 MAPK signal pathway in the expression of cycloxygenase-2 induced by lipopolysaccharide in human bronchial epithelial cells
10.3760/cma.j.issn.1009-6906.2013.06.005
- VernacularTitle:p38丝裂原活化蛋白激酶信号通路在脂多糖调节人支气管上皮细胞环氧化酶-2表达中的作用
- Author:
Ming-ke WANG
1
;
Shuang-hong CHEN
;
Xiong-li XU
;
Wen-bin WU
;
Jian-bo BA
;
Yong-hua TAO
Author Information
1. 海军医学研究所舰艇卫生研究室,上海,200433
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharide;
Human bronchial epithelial cells;
Cycloxygenase-2;
SB203580
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2013;20(6):372-376
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of lipopolysaccharide (LPS) on the expression of cycloxygenase-2 (COX-2) in human bronchial epithelial cells (BEAS-2B),and the role of p38 mitogen-activated protein kinase (MAPK) signal pathway in the expression of COX-2 induced by LPS.Methods Human BEAS2B cells were treated for 2 hours with different concentrations of LPS (0,0.1,1,10,100 mg/L),and the expression levels of COX-2 were monitored with RT-PCR and dose-effect experiment was performed accordingly.Human BEAS-2B cells were treated with 10 mg/L LPS for 0,2,6,12 and 24 hours,and the expression levels of COX-2 were monitored with RT-PCR and dose-effect experiment was also performed.BEAS-2B cells were pretreated with 20 μmol/L SB203580 (p38 MAPK inhibitor) for 1 hour,and then exposed to 10 mg/L LPS for 2 hours.Effect of SB203580 on the expression of COX-2 was detected with RT-PCR.Results COX-2 expression tended to increase,when different concentrations of LPS treated BEAS-2B cells for 2 hours,with the expression level being obviously dose-dependent.After BEAS-2B cells were treated for 2 hours with 10 mg/L LPS,COX-2 expression reached peak,then decreased,and finally came to the basal level at 24 h.And no statistical significance could be noted,when it was compared with that of the control group (P ＜ O.05).p38 MAPK inhibitor,SB203580,could partially inhibit COX-2 expression induced by LPS in BEAS-2B cells.Conclusions LPS could induce the COX-2 expression in BEAS-2B cells in a dose-dependent manner,and the COX-2 expression reaches the peak at 2 h.p38 MAPK is involved in the COX-2 expression induced by LPS in BEAS-2B cells.
