Atomic Force Microscopy Pulling Reveals the Conformation of Metalloproteinase ADAMTS13
10.16156/j.1004-7220.2025.02.026
- VernacularTitle:原子力显微镜拉伸技术揭示金属蛋白酶ADAMTS13的构象
- Author:
Huiting GU
1
;
Junxian YANG
;
Jinhua FANG
;
Jianhua WU
;
Jiangguo LIN
Author Information
1. 华南理工大学 生物科学与工程学院,广州 510006
- Publication Type:Journal Article
- Keywords:
ADAMTS13;
atomic force microscopy;
conformational state;
rupture force;
molecular contour length
- From:
Journal of Medical Biomechanics
2025;40(2):441-448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the conformational states of ADAMTS13 under different pH conditions.Methods Atomic force microscopy(AFM)was applied to pull ADAMTS13 molecules with two distinct pulling systems:the Biotin-Streptavidin system and the 6×His-Anti-His antibody system.The rupture forces and molecular contour lengths were analyzed.Results Under pH 7.4 condition,when ADAMTS13 was pulled by Biotin-Streptavidin system,the mean molecular contour length was(30.93±1.56)nm,exhibiting a bimodal frequency distribution with peak positions at(22.12±0.01)and(49.57±0.05)nm.When ADAMTS13 was pulled using 6×Hist-anti-His antibody system,the mean molecular contour length was(32.77±0.72)nm,also showing a bimodal distribution,with a peak position at(25.73±0.16)and(43.84±0.63)nm,respectively.Under pH 6.0 condition,when ADAMTS13 was pulled by Biotin-Streptavidin system,the mean molecular contour length increased to(47.07±1.6)nm,and the frequency distribution shifted to trimodal,with peak positions at(22±1.25),(55.09±2.62)and(76.69±3.06)nm.The conformation of ADAMTS13 was more extended at pH 6.0 compared with that at pH 7.4.Conclusions ADAMTS13 exists in'closed'and intermediate conformational states at physiological pH 7.4.However,at pH 6.0,ADAMTS13 can adopt'closed',intermediate,and'open'conformational states.This study contributes to a further understanding of the role of ADAMTS13 in normal physiology and thrombotic thrombocytopenic purpura,providing insights for the development of novel recombination ADAMTS13 drugs.
