Biological characteristics of two strains of clinical mucoid Pseudomonas aeruginosa isolates
10.11816/cn.ni.2025-250712
- VernacularTitle:两株黏液型铜绿假单胞菌临床分离株生物学特性
- Author:
Lingli AI
1
;
Hao XIE
;
Lingfei HU
;
Dongsheng ZHOU
;
Huiying YANG
;
Zhengling SHANG
Author Information
1. 贵州医科大学基础医学院免疫学教研室,贵州贵阳 561113
- Publication Type:Journal Article
- Keywords:
Mucoid Pseudomonas aeruginosa;
Clinical isolate;
Drug resistance;
Virulence factor;
Pathogenicity
- From:
Chinese Journal of Nosocomiology
2025;35(17):2608-2612
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE T o compare the biological characteristics,drug resistance and pathogenicity between two strains of mucoid Pseudomonas aeruginosa and the standard strain PAO1.METHODS The strains were identified,and biofilms were detected by 96-well plates method.The bacterial drug resistance was detected by fully automatic drug susceptibility analysis system,the expression levels of RNA of virulence factors were detected by real-time fluorescent quantitative polymerase chain reaction(RT-PCR);the models of rats with pneumonia infection were established through liquid aerosol lung delivery method,the survival status of the rats was observed,and the lev-els of cytokines in bronchoalveolar lavage fluid(BALF)were detected.RESULTS NY4593,NY4605 and PAO1 strains were successfully isolated and identified.NY4593 and NY4605 showed high-yield biofilms,while PAO1 showed low-yield biofilms.The drug resistance rates of NY4593 and NY4605 were remarkably higher than those of the PAO1.The expression levels of exoT and exoY gene RNA of the NY4593 and NY4605 strains were higher than those of the PAO1 strains(P＜0.05);the expression level of exoS gene RNA of the NY4605 was lower than that of the PAO1(P＜0.05).Under the same infection dose,the PAO1 showed more powerful pathogenicity,and the secretion volumes of inflammatory factors interleukin-6(IL-6),interleuki-1β(IL-1β)and interleukin-17A(IL-17A)of the PAO1 were(2858.00±150.30)pg/ml,(7821.00±761.20)pg/ml and(1079.00±225.40)pg/ml respectively,remarkably higher than those of the NY4593 and NY4605(P＜0.05).CONCLUSION The clini-cal mucoid NY4593 and NY4605 remarkably differ from PAO1 in biology and pathogenicity.The study may facilitate deep understanding of the mechanisms of PA infection and provide guidance for clinical treatment,prevention and control.
