Development and clinical diagnostic efficacy of a novel LAMP method tar-geting the tcdC gene in Clostridioides difficile
10.12138/j.issn.1671-9638.20255458
- VernacularTitle:基于艰难梭菌tcdC基因的新型LAMP检测方法的研发及临床诊断效能评估
- Author:
Yuanyuan XIAO
1
;
Juping DUAN
;
Jingxiang ZHOU
;
Qin HUANG
;
Yan QING
;
Haibo WANG
;
Anhua WU
;
Chunhui LI
Author Information
1. 中南大学湘雅医院医院感染控制中心,湖南 长沙 410008
- Publication Type:Journal Article
- Keywords:
Clostridioides difficile;
Clostridioides difficile infection;
molecular diagnosis;
toxin gene;
viru-lence regulatory gene;
tcdC
- From:
Chinese Journal of Infection Control
2025;24(4):451-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for rapidly identifying Clostridioides difficile(C.difficile)and de-termining high-producing toxin strains,conduct clinical evaluation.Methods The loop-mediated isothermal amplifi-cation(LAMP)method was used to identify C.difficile based on the tcdC,tcdA,and tcdB genes.The sensitivi-ty,specificity,and overall consistency of the detection method were evaluated.Results Feces specimens from 499 hospitalized patients suspected of C.difficile-associated diarrhea were detected,with C.difficile detection rate of 12.8％(64/499),out of which the detection rate of toxin-producing C.difficile was 10.8％(54/499).The sensi-tivity,specificity,positive predictive value,and negative predictive value of the detection method for tcdA were 87.2％,98.9％,89.1％,and 98.6％,respectively,and 88.2％,99.6％,90.0％,and 98.73％for tcdB,respec-tively.The total toxin levels of different strains were different,but the average toxin production level of A+B+strains(1.79 μg/mL)was higher than those of A-B+strains(0.72 μg/mL)and A-B-strains(＜0.10 μg/mL).Conclusion The portable high-throughput LAMP detection method can rapidly and efficiently identify C.difficile and determine high-producing toxin strains.
