Analysis of the Relationship Between the Expression of CYP2W1 and miR-491-5p in Hepatocellular Cancer Tissues and Their Correlation With Clinical Pathological Characteristics and Prognosis
10.3969/j.issn.1009-6604.2025.10.002
- VernacularTitle:肝癌组织CYP2 W1、miR-491-5p表达与临床病理特征的关系和预后的相关性分析
- Author:
Wei ZHANG
1
;
Ru YANG
;
Jun SONG
;
Yeben QIAN
Author Information
1. 铜陵市人民医院急诊外科,铜陵 244000
- Publication Type:Journal Article
- Keywords:
Cytochrome P450 family 2 subfamily W member 1;
microRNA-491-5p;
Hepatocellular cancer
- From:
Chinese Journal of Minimally Invasive Surgery
2025;25(10):584-589
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between the expression of cytochrome P450 family 2 subfamily W member 1(CYP2W1)and microRNA-491-5p(miR-491-5p)in hepatocellular cancer(HCC)tissues and clinical pathological characteristics and prognosis.Methods One hunderd and twenty HCC patients who underwent surgical treatment in our hospital from February 2019 to February 2021 were selected as the observation subjects,and the HCC tissues and adjacent tissues specimens were collected during the surgical process.The quantitative real-time PCR(qRT-PCR)was used to detect mRNA expression levels and the immunohistochemistry was used to detect protein expression levels.The differences in miR-491-5p and CYP2W1 mRNA expression between HCC tissues and adjacent tissues,as well as the correlation between their expression and clinical pathological characteristics of patients,were analyzed.The Kaplan-Meier survival analysis curves were performed.Results The expression level of CYP2W1 mRNA in HCC tissues was significantly higher than that in adjacent tissues,and the expression level of miR-491-5p was significantly lower than that in adjacent tissues(P<0.05).The positive expression rate of CYP2W1 protein in HCC tissues was significantly higher than that in adjacent tissues(χ2=23.721,P<0.001).The expression levels of miR-491-5p and CYP2W1 mRNA in HCC tissues were negatively correlated(r=-0.538,P<0.001).The expression of CYP2W1 and miR-491-5p was associated with TNM staging,tumor differentiation,lymph node metastasis,and pathological grading in HCC(P<0.05).The Kaplan-Meier survival curves analysis revealed that the 3-year cumulative survival rate in the CYP2W1 positive-expression group was 31.4%,which was lower than that in the CYP2W1 negative-expression group(63.8%)(log-rank χ2=12.243,P=0.000).The 3-year cumulative survival rate in the miR-491-5p high-expression group was 61.3%,which was significantly higher than that in the miR-491-5p low-expression group(37.9%)(log-rank χ2=4.490,P=0.034).The expression level of CYP2W1 mRNA in the recurrent group was higher than that in the non-recurrent group,and the expression level of miR-491-5p was lower than that in the non-recurrent group(P<0.05).The expression level of CYP2W1 mRNA in the metastatic group was higher than that in the non-metastatic group,and the expression level of miR-491-5p was lower than that in the non-metastatic group(P<0.05).Conclusion CYP2W1 is highly expressed while miR-491-5p is lowly expressed in HCC tissues,and both are significantly associated with certain clinicopathological features and prognosis of HCC patients.
