Transcriptomic analysis and characterization of brain tissue from lambs infected with Enterococcus faecalis
10.16303/j.cnki.1005-4545.2025.10.14
- VernacularTitle:粪肠球菌感染羔羊脑组织的转录组学分析与鉴定
- Author:
Pengfei ZHAO
1
;
You WU
1
;
Longling JIAO
1
;
Ming ZHOU
1
;
Yongjian LI
1
;
Runze ZHANG
1
;
Yayin QI
1
;
Jingjing REN
1
Author Information
1. 石河子大学动物科技学院,新疆石河子 832003
- Publication Type:Journal Article
- Keywords:
Enterococcus faecalis;
blood-brain barrier;
circRNA;
transcriptomics;
lamb
- From:
Chinese Journal of Veterinary Science
2025;45(10):2196-2205
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the pathogenesis of meningitis in lambs caused by Enterococcus faecalis(E.faecalis),and to elucidate the pathogenesis of meningitis from the circRNA level,E.faecalis(named XJ6)was reintroduced into the host to construct a lamb brain tissue injury model,and then the lamb brain tissues were selected for whole genome transcript sequencing at four time in-tervals(24,48,60 and 72 h).Based on the transcriptomics sequencing results,the differential cir-cRNAs were preliminarily screened,and the accuracy of the transcriptomics data was verified by real-time fluorescence quantification.The results showed that the experiments successfully con-structed the brain tissue injury model of E.faecalis infected lambs,and the GO enrichment analy-sis results showed the most significant 10 cellular components,10 biological processes,and 10 mo-lecular functions.KEGG pathway enrichment results revealed that the genes targeted by differenti-al circRNAs were mainly focused on signaling pathways regulating the neuronal cells,the MAPK signaling pathway,the Rap1 signaling pathway,VDEF signaling pathway,Apelin signaling path-way,vascular smooth muscle contraction,and cancer development.The differentially expressed cir-cRNAs validated by real-time quantitative PCR were completely consistent with the transcriptomic results.Among them,novel_circ_0004872 was identified as a candidate circRNA potentially involved in altering the permeability of the blood-brain barrier in lambs,providing a theoretical ref-erence for exploring the regulatory role of circRNAs in blood-brain barrier function.
