Circadian rhythm expression of bmal1, clock, cry1, per1 in peripheral white blood cells of mouse
10.3760/cma.j.issn.1007-6239.2014.04.011
- VernacularTitle:小鼠外周血白细胞节律基因bmal1、clock、cry1、per1表达的近日节律性研究
- Author:
Qingmin WANG
1
;
Ying TANG
1
;
Jun SHEN
1
;
Qiuhong LIU
1
;
Fenzhou SHI
1
;
Kehua LI
1
;
Shenglong DAI
1
Author Information
1. 海军医学研究所航空医学研究室,上海,200433
- Publication Type:Journal Article
- Keywords:
Mice;
Circadian rhythm;
Leukocytes;
Gene expression;
Polymerase chain reaction
- From:
Chinese Journal of Aerospace Medicine
2014;25(4):275-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate circadian rhythm of four genes (bmal1,clock,cry1,per1) in mouse peripheral white blood cells under the 12 h-light and 12 h-dark cycle (12L/12D) condition.Methods Forty-eight male mice were kept under the light regime of 12L/12D for four weeks.According to random number table,they were randomly and averagely divided into 6 groups.Then,the blood samples were taken and white blood cells were separated every four hours in a circadian day (9:00,13:00,17:00,21:00,1:00 and 5:00).The total RNA was extracted from the white blood cells and the mRNA level of each gene was quantified by real-time polymerase chain reaction (real-time PCR).The data were fitted by cosine function for getting the rhythm.The difference between apex and trough was analyzed by one-ANOVA.Results Under the light regime of 12L/12D,the four genes in peripheral white blood cells showed a significant circadian oscillation with different apex and trough.The apexes of four genes were higher than the troughs (P＜0.01).The equation fitted apex phase of clock gene was at about 5:00,compared to the apex time of the other three genes were at about 13:00-15:00.The apex time of clock mRNA was ahead of brnal1,and the apex and amplitude of it were higher than those of brnal1.However,the mRNAs of cry1 and per1 showed a similar apex time and apex level.Conclusions The findings demonstrate that the expression of the four genes in white blood cells shows an obvious circadian rhythm.It seems that clock plays more important role than bmal1 in the positive regulation,and cry1 and per1 play a similar role in negative regulation.This result will not only be helpful to understand the circadian fluctuation of immune function,but also to provide a target for implementing diagnosis and therapy of immune disorders.Further study is needed to elucidate the control effect of these genes in different immune cells in peripheral blood.
