ERMAP deficiency aggravates IMQ-induced psoriasis-like skin inflammation in mice
10.3969/j.issn.1000-484X.2025.05.002
- VernacularTitle:ERMAP缺失加重IMQ诱导的小鼠银屑病样皮肤炎症
- Author:
Lu XIA
1
;
Wei CHEN
1
;
Yiwen PAN
1
;
Zhihong LIU
1
;
Min SU
1
Author Information
1. 贵州医科大学基础医学院组织学与胚胎学教研室,贵阳 550025
- Publication Type:Journal Article
- Keywords:
ERMAP;
Psoriasis;
Immunoreaction;
Inflammatory factors
- From:
Chinese Journal of Immunology
2025;41(5):1030-1034
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of ERMAP on imiquimod(IMQ)-induced psoriasis-like skin inflammation in mice and its related mechanism.Methods:The experimental mice were divided into 3 groups:Sham group,WT group and ERMAP-/-group,with 9 mice in each group.The Sham group was smeared with Vaseline,and the WT group and ERMAP-/-group were smeared with IMQ to induce psoriatic dermatitis.The severity of IMQ-induced psoriasis lesions in mice were evaluated according to the psoria-sis area and severity index(PASI)and the HE staining pathology score.The expressions of F4/80 and Ki67 in mouse skin lesions were observed by immunofluorescence staining.The relative expressions of IL-1β,IL-6,IFN-γ and iNOS in skin lesions were detected by qRT-PCR.Flow cytometry was used to detect the proliferation and activation of T cells and the proportion of macrophages in spleen.Results:In the IMQ-induced mouse model of psoriasis-like dermatitis,the skin lesions of ERMAP gene knock out mice showed more severe squamous accumulation and skin bulge,more inflammatory cells aggregation and cytokine production,and the proportion of immune cells in the spleen of mice increased compared with the WT group,and the proportion of M1 macrophages increased.Conclu-sion:ERMAP deficiency aggravates IMQ-induced psoriasis-like skin inflammation in mice by enhancing immune response.
