The pathological changes of weightlessness simulation on streptococcus pneumoniae infection to the lung tissue of rats
10.3760/cma.j.issn.1007-6239.2014.03.009
- VernacularTitle:模拟失重大鼠肺组织肺炎链球菌感染的病理改变
- Author:
Minli ZHU
1
;
Ping WANG
1
;
Yan CUI
1
;
Shijie PEI
1
;
Xiaohui WANG
1
Author Information
1. 100101,解放军第三〇六医院
- Publication Type:Journal Article
- Keywords:
Weightlessness simulation;
Streptococcus pneumoniae;
Models,animal;
Rats,Wistar
- From:
Chinese Journal of Aerospace Medicine
2014;25(3):194-197,封4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish bacterial pneumonia model of rats in simulated weightlessness state,and to observe the effects of weightless on streptococcus pneumoniae infection to the lung tissue of rats.Methods Thirty-six male Westar rats were randomly divided into weightlessness and infection (WI) group (n=12),weightlessness and control (WC) group (n=12),and 1 G gravity and infection (GI) group (n=12).On the 8th day after bacterial suspension instilled into trachea,the rats were killed.The leukocytes and neutrophils,C-reactive protein level,CD4+/CD8+ ratios in the peripheral blood were analyzed.The pathology of lung tissue was also observed.Results There were statistical significances of neutrophilic granulocyte count (NEU),neutrophilic granulocyte percentage (NEU％) among three groups (F=6.12,P＜0.01 and F=4.23,P＜0.05 respectively),and the WI group showed the highest values but no statistical difference was found in leukocytes count.The WI group had the highest level of CRP while the WC group had the lowest,and statistical significances were seen among three groups (F=19.49,P＜0.01).But CD4+/CD8+ ratios in peripheral blood among three groups had no statistical differences.The changes of lung pathology in WI group were the most severe.Some of these changes were also visible in WC group and GI group,but the inflammatory response to a lesser extent,and the pathological changes were the least in WC group.Conclusions The immune function of rat would reduce in weightlessness environment.Once the lung was insulted by streptococcus pneumoniae,the pathological changes and inflammatory response become more severe.
