Simultaneous content determination of twelve constituents in Anshen Buxin Liuwei Pills by HPLC-MS/MS and their chemical pattern recognition
10.3969/j.issn.1001-1528.2025.09.002
- VernacularTitle:HPLC-MS/MS法同时测定安神补心六味丸中12种成分的含量及其化学模式识别研究
- Author:
Cheng-dong LIU
1
;
Jun LI
;
Qian ZHANG
;
Jing LIU
;
Jing-kun LU
;
Xin DONG
;
Yuan-hong LIAO
;
Yue-wu WANG
Author Information
1. 内蒙古医科大学药学院,内蒙古呼和浩特 010110
- Publication Type:Journal Article
- Keywords:
Anshen Buxin Liuwei Pills;
chemical constituents;
content determination;
chemical pattern recognition;
HPLC-MS/MS;
cluster analysis;
principal component analysis;
orthogonal partial least square-discriminant analysis
- From:
Chinese Traditional Patent Medicine
2025;47(9):2834-2840
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC-MS/MS method for the simultaneous content determination of dehydrodiisoeugenol,eugenol,costiolactone,dehydrocostiolactone,quercetin,isorhamnetin,luteolin,caffeic acid,gallic acid,protocatechuic acid,ellagic acid and kaempferol in Anshen Buxin Liuwei Pills,and to make chemical pattern recognition.METHODS The analysis was performed on a 35 ℃ thermostatic Shim-pack GST-HP C18 column(2.1 mm × 100 mm,3 μm),with the mobile phase comprising of methanol-water(containing 0.1％formic acid)flowing at 0.25 mL/min in a gradient elution manner,and electron spray ionization source was adopted in positive and negative ion scanning with multiple reaction monitoring mode.Subsequently,cluster analysis,principal component analysis and orthogonal partial least square-discriminant analysis were performed.RESULTS Twelve constituents showed good linear relationships within their own ranges(r≥0.999 0),whose average recoveries were 95.38％-105.00％with the RSDs of 1.91％-5.14％.Thirteen batches of samples were clustered into 3 types,ellagic acid,dehydrocodenolactone,dehydrodiisoeugenol,protocatechuic acid,gallic acid,quercetin and kaempferol were taken as potential quality differential markers.CONCLUSION This accurate,sensitive,stable and reproducible method can be used for the quality control and evaluation of Anshen Buxin Liuwei Pills.
