Chemical constituents from Tetrastigma hemsleyanum and their antitumor activity in vitro
10.3969/j.issn.1001-1528.2025.09.017
- VernacularTitle:金线吊葫芦化学成分及其体外抗肿瘤活性研究
- Author:
Yi LONG
1
;
Li-zhi ZHANG
;
Piao-piao JIANG
;
Nan KUANG
;
Xin-yu ZHANG
;
Yu-pei YANG
;
Bin LI
;
Wei WANG
Author Information
1. 湖南中医药大学药学院,湖南省中医药民族医药国际联合实验室,湖南长沙 410208
- Publication Type:Journal Article
- Keywords:
Tetrastigma hemsleyanum Diels et Gilg;
chemical constituents;
isolation and identification;
antitumor activity
- From:
Chinese Traditional Patent Medicine
2025;47(9):2932-2941
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the chemical constituents from Tetrastigma hemsleyanum Diels et Gilg and their antitumor activity in vitro.METHODS Silica gel,ODS,Sephadex LH-20 and semi-preparative HPLC were used for isolation and purification,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antitumor activity in vitro was determined by MTT mothod.RESULTS Twenty-eight compounds were isolated and identified as triphyllin A(1),eruberin B(2),(2S,4R)-5,7-dihydroxy-4,4'-dimethyl-6,8-dimethyl-flavan-5-O-β-D-6-acetylglucopyranoside-7-O-β-D-glucopyranoside(3),eruberin A(4),abacopterin Ⅰ(5),matteucinol(6),homoerodictyol(7),(2S)-5,3',4'-trihydroxy-7-methoxy-flavanone(8),(2S)-5,2',5'-trihydroxy-7-methoxyflavanone(9),galinsonside B(10),quercetin-3-O-β-D-glucopyranoside(11),kaempferol 3-O-robinobioside(12),rutin(13),geniposide(14),jasminoside A(15),β-sitostenone(16),sitosterol palmitate(17),β-sitosterol(18),ursolic acid(19),hyptadienic acid(20),3,4-dihydroxybenzoic acid(21),3,4-dimethoxybenzoic acid(22),gallic acid(23),dibutylphthalate(24),bis-(2-ethylhexyl)phthalate(25),9-nonadecenoic acid(26),triacylglycerol(27),crocin Ⅰ(28).The IC50 values of compound 1 for human gastric adenocarcinoma cells BGC-823 and human colon cancer cells HCT-116 were(22.07±0.38),(20.67±0.11)μmol/L,respectively.The IC50 value of compound 9 for BGC-823 cells was(21.58±0.05)μmol/L,and the IC50 value of compound 4 for HCT-116 cells was(16.67±0.36)μmol/L.CONCLUSION Compounds 1-10,14-15 and 28 are first isolated from Tetrastigma genus.Compounds 1,4,9 have weak antitumor activity in vitro.
