Investigation of effects of petroleum ether fraction from Derris eriocarpa on glucose and lipid metabolism in a mouse model of metabolic syndrome via ATF3/HNF4ɑ/CYP7A1 pathway
10.3969/j.issn.1001-1528.2025.09.012
- VernacularTitle:基于ATF3/HNF4ɑ/CYP7A1通路探讨毛果鱼藤石油醚提取物对代谢综合征小鼠糖脂代谢的影响
- Author:
Jing YAN
1
;
Jie WENG
;
Xuan ZHANG
;
Xue LI
;
Chao-nan KONG
;
Hong-cun LIU
;
Li-fang YANG
;
Ming-guo JIANG
;
Qiu-yan LIANG
;
Li-ting HE
Author Information
1. 广西民族大学化学化工学院,林产化学与工程国家民委重点实验室,广西林产化学与工程重点实验室/协同创新中心,广西南宁 530006
- Publication Type:Journal Article
- Keywords:
Derris eriocarpa How;
petroleum ether fraction;
metabolic syndrome;
glycolipid metabolism;
lowering blood sugar;
lowering blood lipid
- From:
Chinese Traditional Patent Medicine
2025;47(9):2902-2911
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate effects of petroleum ether fraction from Derris eriocarpa How on glucose and lipid metabolism in a mouse model of metabolic syndrome(MS).METHODS KM mice were fed a high-fat diet and administered streptozotocin intraperitoneally to establish MS models.The MS mice were then randomly assigned to the model group,the metformin hydrochloride group,the lovastatin group,the ursolic acid group,and the high-,medium-and low-dose D.eriocarpa petroleum ether fraction groups,with 10 mice in each group.Ten additional mice maitained on a normal diet served as the normal control group.After 4 weeks of intragastric administration,glucose and lipid metabolism indicators were measured.Hepatic pathological changes were assessed using HE staining and oil red O staining.Liver tissue mRNA expressions of ATF3,PEPCK,FXR,CYP7A1,HNF4ɑ,CYP8B1 and SRB1 were quantified by RT-qPCR.Hepatic protein expressions of ATF3,HNF4ɑ,PEPCK,FXR and CYP7A1 was analyzed by Western blot in MS mice.RESULTS Compared to the model group,the high-dose D.eriocarpa petroleum ether fraction group exhibited significant glucose tolerance improvement(reduced OGTT-AUC,P＜0.01);favorable serum lipid modulation in terms of increased HDL-C levels(P＜0.01)and decreased TG,TC,LDL-C(P＜0.01);reduced renal biomarkers(BUN,SCR)and hepatotoxic indicators of TBA,AST and ALT activities(P＜0.01);alleviated hepatic lipid accumulation and histopathological damage;downregulated mRNA and protein expressions of ATF3,HNF4ɑ and PEPCK,as well as CYP8B1 mRNA expression(P＜0.01);and upregulated mRNA and protein expressions of FXR and CYP7A1,along with SRB1 mRNA expression(P＜0.01).CONCLUSION D.eriocarpa petroleum ether fraction ameliorates glucose and lipid metabolism dysregulation in MS mice by modulating the ATF3/HNF4ɑ/CYP7A1 signaling pathway,consequently eliciting hypoglycemic,hypolipidemic,hepatoprotective and nephroprotective effects.
