Effect of Guanxinning injection on myocardial infarction by regulating cardiac immunity through CCL21
10.12360/CPB202409085
- VernacularTitle:冠心宁注射液通过CCL21调节心脏免疫改善心肌梗死作用
- Author:
Yu-xin BAI
1
;
Ying-xue ZHANG
;
Ting-ting SHI
;
Si-nan ZHU
;
Zhen-kun XU
;
Hong WANG
;
Lu CHEN
Author Information
1. 天津中医药大学测试中心,天津 301617
- Publication Type:Journal Article
- Keywords:
Guanxinning injection;
myocardial infarc-tion;
immunity;
CCL21;
lymphatic;
T cells
- From:
Chinese Pharmacological Bulletin
2025;41(5):960-969
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the mechanism of Guanxinning injection regulating cardiac immune mi-croenvironment to improve myocardial infarction in mice.Methods In this study,MI model was estab-lished by permanent ligation of left anterior descending coronary artery in mice.The mice were divided into five groups:sham operation group,model group,Guanxinning injection low dose group,Guanxinning in-jection high dose group and positive drug captopril group.Hearts were weighed,heart tissues were collect-ed,and Masson staining was used for pathological anal-ysis of heart tissues;immunofluorescence staining was used to detect apoptosis and CCL21 expression in the infarct border zone;flow cytometry was used to detect the proportion of immune cells in myocardial ischemia tissues and lymph nodes;PCR was used to detect CCL21 expression in heart and in vitro human lymphat-ic endothelial cells(HLEC).Results Compared with the model group,the low and high dose groups of Guanxinning injection significantly improved cardiac hypertrophy.Apoptosis in the border zone of myocardi-al infarction was reduced in the low and high dose groups of Guanxinning injection and captopril group.Compared with the model group,the proportion of leu-kocytes in the infarct border zone was dreduced and the proportion of CD4+T cells,Treg cells,and CD8+T cells in the mediastinal lymph nodes and infarct border zone of the heart was regulated in the low and high dose groups of Guanxinning injection;CCL21 secretion by the heart and lymphatic vessels increased.Conclu-sions Guanxinning injection can significantly improve cardiac hypertrophy and fibrosis in MI mice,reduce ap-optosis in the infarct border zone,and play a role in an-ti-myocardial ischemia injury by promoting CCL21 ex-pression in lymphatic vessels to regulate the proportion of mediastinal lymph nodes and cardiac T cells after myocardial infarction.
