Generation,identification and clinical significance of rabbit polyclonal anti-body against human KHSRP-Acetyl-K205
10.3969/j.issn.1000-484X.2025.05.032
- VernacularTitle:兔抗人源KHSRP-Acetyl-K205多克隆抗体制备、鉴定及临床意义
- Author:
Lei WANG
1
;
Renjie CAI
;
Guangyu TIAN
;
Ming XU
Author Information
1. 扬州大学附属江都人民医院肿瘤科,扬州 225200
- Publication Type:Journal Article
- Keywords:
KHSRP;
Acetylation;
Rabbit polyclonal antibody;
Tumor
- From:
Chinese Journal of Immunology
2025;41(5):1215-1219
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To generate a rabbit polyclonal antibody specific for human acetylated KHSRP and to evaluate its poten-tial value and clinical significance in detecting colorectal cancer samples.Methods:An acetylated peptide Lys205(K205)of KHSRP protein was designed and synthesized,which was coupled to KLH at its carboxyl terminus to form an antigen-carrier conjugate.After five times immunization in New Zealand rabbits,blood serum was separated to determine antibody titer by indirect ELISA.Polyclonal antibody by affinity chromatography method was purified,and specificity of antibody was determined by Western blot and immunohis-tochemistry(IHC).Results:Final rabbit serum antibody ELISA titer was>5.12×105,which could be used for specific detection of K205-acetylated KHSRP protein by Western blot and IHC.Conclusion:Role of KHSRP in tumor may be closely related to its surface acetylation modification.A rabbit polyclonal antibody against human acetylated KHSRP has been successfully generated,and an excel-lent foundation for assessing relationship between acetylated KHSRP and tumor processes in cancer diseases has been made.
