Effect of Xiaotanjieyu recipe on the proliferation and apoptosis of MCF-10AT cells and the expression of PTEN protein and mRNA before cancerous lesion in breast cancer
10.3760/cma.j.issn.1009-6906.2017.01.010
- VernacularTitle:消痰解郁方对乳腺癌前病变MCF-10AT细胞增殖与凋亡及对PTEN蛋白和mRNA表达的影响
- Author:
Jing ZHAO
1
;
Pinkang WEI
;
Lijuan XIU
;
Jianpeng JIAO
;
Chaoqin YU
Author Information
1. 第二军医大学附属长征医院中医科, 上海,200003
- Publication Type:Journal Article
- Keywords:
Xiaotanjieyu recipe;
MCF-10AT;
Precancerous lesions of breast cancer;
PTEN protein
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2017;24(1):41-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of Xiaotanjieyu recipe (XTJYR)in the inhibition of precancerous lesion of breast cancer through the study of its effects on the growth and apoptosis of MCF-10AT cells,as well as its effects on expressions of PTEN protein and mRNA.Methods Changes in the inhibition rate of MCF-10 AT cells growth in precancerous lesion of breast cancer after medication were closely observed at hour 24 and 48,by using CCK-8 method in the 3 groups,i.e.the herbal medicine group (or the XTJYR group),the western medicine group (or the tamoxifen group) and the control group.Western blot was used to detect changes in the expression of PTEN,and Q-PCR method was employed to detect the expression of PTEN and mRNA in the 3 groups following intervention.Results (1) OD values of the XTJYR group and the tamoxifen group were all lower than those of the control group,with statistical significance (P ＜ 0.05).The inhibition was time-dependent,furthermore,the inhibition rate of the XTJYR group was higher than that of the tamoxifen group,and statistical significance could be noted,when comparisons were made between them (P ＜ 0.05).(2) At hour 48 after drug intervention,the rates of apoptosis for the XTJYR group and the tamoxifen group were respectively (25.40 ±4.69)％ and (16.93 ± 1.29)％,which were all significantly higher,as compared with those of the control group (2.63 ±0.29％),also with statistical significance (P ＜0.05).The apoptosis rate of the XTJYR group was higher than that of the tamoxifen group and statistical significance could be seen,when comparisons were made between them (P ＜ 0.05).(3) The expression levels of PTEN were significantly increased in both the XTJYR group and the tamoxifen group 48 hours after intervention on MCF-10AT cells,and the PTEN expression level of the XTJYR group was greater than that of the tamoxifen group.PTEN mRNA expression level of the XTJYR group was (1.31 ±0.04),while that of the tamoxifen group was 0.61 ±0.11,which were all higher than that of the control group (0.13 ±0.13),and the expression level of the XTJYR group was higher than that of the tamoxifen group.Statistical significance could be found,when comparisons were made between them (P ＜ 0.05).Conclusions Xiaotanjieyu recipe proved to have the effects of inhibiting proliferation and promoting apoptosis,the mechanism of which might be related with the upregulation of PTEN protein and the elevated level of gene expression.
