Research on the efficacy of the composite gel collagen from marine fish skin on stomatocace in rats
10.3760/cma.j.issn.1009-6906.2017.01.005
- VernacularTitle:海洋鱼皮胶原蛋白复合物凝胶制剂对大鼠口腔溃疡的疗效研究
- Author:
Sheng JIANG
1
;
Dagui CHEN
;
Tiejun LI
;
Yibo WEI
;
Xiaogang XU
Author Information
1. 第二军医大学, 上海,200433
- Publication Type:Journal Article
- Keywords:
Collagen from marine fish skin;
Gel;
Stomatocace;
Therapeutic efficacy
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2017;24(1):21-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the therapeutic efficacy of the composite gel collagen from marine fish skin on stomatocace in rats.Methods Fifty-six male SD rats were randomly divided into 7 groups:i.e.the 3 dosages groups (the high dosage,medium dosage and low dosage groups),the single gel group,the single collagen group,the positive control group and the model group,each consisting of 8 animals.Thirty-five per cent glacial acetic acid solution was used to develop the rat model of stomatocace,then,changes in stomatocace sizes were observed and detected both before and after medication in all the groups.Changes in the ultrastructure of the ulcer-affected cells in various groups were observed under electronmicroscope and pathological changes in the surface area of ulcer-affected tissues in various groups were further observed by HE staining.Results The composite gel collagen from marine fish skin could reduce ulcer sizes of the affected surface areas in various dosage groups.At day 3 after medication,ulcer sizes in the high dosage,medium dosage and low dosage groups as well as the model group were respectively (8.87 ± 2.11) mm2,(9.50 ± 0.48)mm2,(9.62 ± 1.29) mm2 and (12.31 ± 0.69) mm2.At day 7 after medication,ulcer sizes in the high dosage,medium dosage and low dosage groups as well as the model group were respectively (6.13 ± 2.00) mm2,(7.31 ± 2.70) mm2,(8.92 ± 2.09) mm2 and (10.46 ± 1.33) mm2.At day 10 after medication,ulcer sizes in the high dosage,medium dosage and low dosage groups as well as the model group were respectively(2.52 ± 1.30) mm2,(3.42 ± 1.80) mm2,(4.65 ± 1.51) mm2 and (8.62 ± 1.24) mm2.At day 12 after medication,ulcer sizes in the high dosage,medium dosage and low dosage groups as well as the model group were respectively(0.44 ± 0.92) mm2,(0.41 ± 0.40) mm2,(0.87 ± 1.20) mm2 and (3.12 ± 1.71) mm2.Statistical significance could be noted,when comparisons were made between different dosages groups and the model group (P ＜ 0.05),furthermore,there was a certain dosage-dependent relationship between them.Electronmicroscopy revealed that the tissues of ulcer-affected areas in various dosage groups were generally healed,epithelial cells were tightly lined and degenerative cells could only be occasionally seen between epithelial cells,but without presence of inflammatory cells.Pathological observation revealed that lesion of the tissues in the ulcer-affected areas of various dosage groups was relatively lighter and tissue necrosiswas also relatively lighter.Furthermore,granulation and fibrosis of the affected tissues were quite obvious.Conclusions Composite gel collagen from marine fish skin could produce certain therapeutic efficacy on stomatocace in rats.
