Comparative study on the resistance of phage and bacteria indicative of the disinfection effect on object surfaces
10.3760/cma.j.cn112150-20241230-01062
- VernacularTitle:噬菌体与物体表面消毒效果指示菌的抵抗力对比研究
- Author:
Cuicui MA
1
;
Qi ZHANG
;
Ying TONG
;
Li YU
;
Qiongqiong FANG
;
Baoying ZHANG
;
Jin SHEN
Author Information
1. 北京市疾病预防控制中心，消毒与有害生物防制所，北京 100013
- Publication Type:Journal Article
- Keywords:
phage Phi-X174;
Disinfection effect;
Indicator microorganism;
Resistance
- From:
Chinese Journal of Preventive Medicine
2025;59(10):1703-1707
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the differences in resistance to chemical disinfectants between bacteriophage Phi-X174 and disinfection effect indicator microorganisms, Staphylococcus aureus and Escherichia coli. Methods:Three commonly used disinfectants, including chlorine-containing disinfectants, alcohol-based disinfectants and quaternary ammonium salt disinfectants, were selected to analyze the differences in resistance of phage Phi-X174, Staphylococcus aureus and Escherichia coli by using the quantitative sterilization test of suspension. Results:The 250 mg/L sodium dichloroisocyanurate solution treated for 10 minutes yielded log reduction values of 3.39, 7.25 and 7.37 against phage Phi-X174, Staphylococcus aureus and Escherichia coli respectively. The 40% ethanol for 1 minute yielded log reduction values of 0.03, 2.46 and 7.30 against phage Phi-X174, Staphylococcus aureus and Escherichia coli, respectively. The 100 mg/L benzalkonium chloride for 10 minutes yielded log reduction values of 1.41, 6.84 and 0.93 for phage Phi-X174, Staphylococcus aureus and Escherichia coli, respectively. Conclusion:Phage Phi-X174 has stronger resistance to chlorine-containing disinfectants and alcohol-based disinfectants than Escherichia coli and Staphylococcus aureus. Its resistance to quaternary ammonium salt disinfectants is between that of Staphylococcus aureus and Escherichia coli.
