Plasmid characteristics and genome tracing analysis of a bacterial dysentery outbreak in Shandong Province, originating from Shigella sonnei producing extended spectrum β-lactamase
10.3760/cma.j.cn112150-20240626-00506
- VernacularTitle:山东省1起细菌性痢疾疫情来源产超广谱β-内酰胺酶宋内志贺氏菌的质粒特征及基因组溯源分析
- Author:
Shuang WANG
1
;
Lu LIU
;
Yu MA
;
Hui LYU
;
Xiaolin YU
;
Ziqing LIU
;
Yuzhen CHEN
;
Ming FANG
;
Yi LIU
;
Gaoxiang SUN
;
Yanru CHEN
;
Lianchen FU
;
Zengqiang KOU
Author Information
1. 山东省疾病预防控制中心传染病防制所，济南 250014
- Publication Type:Journal Article
- Keywords:
Bacterial dysentery;
Shigella sonnei;
Outbreak of epidemic;
Genomic sequencing;
Plasmid characteristics
- From:
Chinese Journal of Preventive Medicine
2025;59(6):901-907
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the drug resistance gene characteristics, plasmid characteristics and genome tracing of Shigella sonnei causing a bacillary dysentery outbreak in Shandong Province. Methods:Sixty-five Shigella sonnei strains isolated from a 2021 outbreak in a county of Shandong Province were analyzed using antimicrobial susceptibility testing, whole genome sequencing (WGS), characterization of resistance and virulence genes, plasmid profiling, core genome multilocus sequence typing (cgMLST), and single nucleotide polymorphism (SNP) analysis. Results:All isolates had the same resistance phenotype and genotypes and were multidrug-resistant ESBL-producing Shigella sonnei, carrying important virulence genes. Plasmid analysis revealed a conserved genetic arrangement, pil( M/ N/ O2/ P)-tra( F/ H/ J/ K/ N/ O/ P/ Q)-IS Ecp1- blaCTX-M-14-Tn 903- yub( J/ I/ F/ G/ E/ D), and shared across strains from diverse regions and bacterial species. The cgMLST and SNP analyses demonstrated concordant clustering, with all 65 outbreak-related strains forming a single cluster alongside human-derived strains from Guangxi. Conclusion:The ESBL-producing Shigella sonnei responsible for the outbreak shares a homologous relationship with Guangxi human-derived strains, and the detected resistance plasmids and virulence genes underscore the need to strengthen drug resistance surveillance and genome tracing.
