Treatment and mechanism of chrysoeriol on pulmonary hypertension based on network pharmacology and experimental study
- VernacularTitle:基于网络药理学及实验研究金圣草黄素对肺动脉高压的治疗和作用机制
- Author:
Ying-fang MA
1
;
Meng CAI
1
;
Dan FENG
1
;
Yang GUO
1
;
Yu-he TIAN
1
;
Yun-hua ZHANG
1
;
Li-li WEI
1
;
Yang WANG
1
;
Jun-qiang SI
1
Author Information
- Publication Type:Journal Article
- Keywords: Chrysoeriol; pulmonary arterial hyperten-sion; molecular docking; network pharmacology; vas-cular remodeling; pulmonary artery smooth muscle cells
- From: Chinese Pharmacological Bulletin 2025;41(11):2167-2176
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effect of chrysoeriol on pulmonary vascular remodeling in pulmonary hyper-tension by animal experiments combined with cell ex-periments,and to explore its potential therapeutic tar-gets by network pharmacology.Methods The target of chrysoeriol was collected in Targetnet,SEA and SwissTargetPrediction database.Pulmonary arterial hy-pertension(PAH)targets were collected in the Dis-GeNET and GeneCards databases,and PPI network map was drawn in the STRING database,and key tar-gets were screened.The GO and KEGG pathway en-richment analysis was carried out through DAVID data-base and Weishengxing platform.AutoDock software was used for molecular docking of key core targets.The PAH model of rats was constructed,and the pulmo-nary hemodynamics and vascular remodeling were de-tected by echocardiography,HE and Masson staining.Primary pulmonary smooth muscle cells were extracted,and the effects of drugs on pathway proteins were de-tected in vitro.Results The results of network phar-macology showed that chrysoeriol exerted therapeutic effects on pulmonary hypertension by affecting key tar-gets such as AKT1,SRC,EGFR,MMP9 and gsk3 β,and signaling pathways such as EGFR and PI3K-AKT.Molecular docking showed that chrysoeriol had good binding ability with 5 key target genes.Animal experi-ments showed that the pulmonary hemodynamic func-tion of PAH rats was significantly improved after ad-ministration of chrysoeriol.The remodeling of small pulmonary arteries was significantly reduced.Cell ex-periments showed that chrysoeriol could inhibit the ex-pression of proliferation,migration and phenotypic transformation genes.Conclusion Chrysoeriol may play a role in the treatment of pulmonary hypertension through multiple targets.
