Biological characteristics of Salmonella enteritidis bacteriophage JD01 and its potential application in sterilization of contaminated liquid eggs
10.3760/cma.j.cn112309-20240710-00255
- VernacularTitle:肠炎沙门菌噬菌体JD01的生物学特性及在污染蛋液中的杀菌潜在应用
- Author:
Yao MENG
1
;
Jinghua LI
1
;
Xin HUANG
1
;
Zhaoxue LI
1
;
Honglan HUANG
1
;
Lingling SUN
1
;
Chunyan ZHAO
1
Author Information
1. 吉林大学基础医学院病原生物学系，长春 130021
- Publication Type:Journal Article
- Keywords:
Salmonella enteritidis;
Bacteriophage;
Biological control agent;
Genomic analysis
- From:
Chinese Journal of Microbiology and Immunology
2024;44(12):1048-1058
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate and identify a phage that can specifically lyse Salmonella enteritidis from the environment, and provide a theoretical and research basis for its use in food safety prevention and control through the exploration of the biological characteristics of the phage and the efficacy of lysing bacteria. Methods:The double-layer agar method was applied to isolate phages, and the pH stability, thermal stability, host range, one-step growth curve of the phage were determined. The phage morphology was observed by transmission electron microscopy. Next-generation sequencing was performed for the phage genome, and the lysis efficiency was evaluated.Results:Salmonella enteritidis phage JD01 had a (58±3) nm icosahedral head, a (120±5) nm tail, a thin bottom plate of about 17 nm and 5-6 spines at the tail end. The genome of phage JD01 was composed of 44 880 bp with a GC content of 50.17%, and it contained 63 putative open reading frames without any tRNA genes. The genome comparison and phylogenetic analysis revealed that phage JD01 was a novel phage belonging to the class Caudoviricetes, the family Guernseyvirinae, and the genus Jerseyvirus, lacking virulence genes and drug resistance genes. It survived below 70℃ and pH4.0-12.0, and the lysed bacteria had genus specificity. The phage JD01 had a good bactericidal effect on Salmonella enteritidis in contaminated liquid eggs. Conclusions:Phage JD01 has strong lytic activity and a wide host range, and has great potential application in food safety and control of Salmonella enteritidis pollution, which can be considered for the development of food safety biological control agents.
