Total triterpenoids from Hovenia dulcis increase sensitivity of A549/DDP to cisplatin by inducing Nrf2-mediated ferroptosis
- VernacularTitle:枳椇总三萜通过诱导Nrf2介导的铁死亡增强A549/DDP细胞对顺铂的敏感性
- Author:
Xiao-lan KUANG
1
;
Dong-ning SHEN
;
Ting FU
;
Fan WU
;
Jian-zhan YANG
;
Jin-lang ZHONG
;
Bo LIU
;
Fang-fang XU
Author Information
- Publication Type:Journal Article
- Keywords: total triterpenoids from Hovenia dulcis; drug-resistant; non-small cell lung cancer; ferroptosis; apoptosis; Keap-1/Nrf2/HO-1; Nrf2-GPX4
- From: Chinese Pharmacological Bulletin 2025;41(11):2128-2134
- CountryChina
- Language:Chinese
- Abstract: Aim To elucidate the underlying mecha-nism by which total triterpenoids extracted from Hove-nia dulcis(H-TP)enhance the sensitivity of A549/DDP cells to cisplatin.Methods The ARE-Nrf2 lu-ciferase reporter assay was applied to investigate the impact of H-TP on Nrf2 expression.Western blot was used to detect the protein levels of Keap-1/Nrf2/HO-1,Nrf2-GPX4 signaling pathway,apoptosis-related proteins of Bcl-2 and Bax.Further validation of its effects on Nrf2 was conducted by using Nrf2 activator/inhibitor.Results H-TP could enhance the sensitivi-ty of A549/DDP cells to cisplatin by modulating the expression of apoptosis-related proteins Bax and Bcl-2,inhibiting the Keap-1/Nrf2/HO-1/GPX4 signating pathway in A549/DDP cells,and inducing ferroptosis.Conclusion H-TP enhances the sensitivity of A549/DDP cells to cisplatin by inducing the Nrf2-mediated ferroptosis pathway.
