Effect of salidroside combined with rosavin on ischemic brain injury in rats
- VernacularTitle:基于蛋白质组学探讨红景天苷联合络塞维对缺血性脑卒中大鼠的作用机制
- Author:
Wen-fang LAI
1
;
Yu-ting JIANG
;
Jing-quan CHEN
;
Xue-rui ZHENG
;
Hui-ling WU
;
Qing-qing WU
;
Yan CHEN
;
Ya LIN
Author Information
- Publication Type:Journal Article
- Keywords: salidroside; rosavin; ischemic stroke; STEP/CRTC1 signaling pathway; apoptosis
- From: Chinese Pharmacological Bulletin 2025;41(11):2058-2065
- CountryChina
- Language:Chinese
- Abstract: Aim To study the mechanism of salidro-side combined with rosavin in rats with ischemic stroke.Methods The MCAO rats was established by using thread-embolic method.The rats were divided into the sham group,MCAO group,salidroside com-bined with rosavin group,and positive control group;the drug was given continuously for seven days.Western blot was used to detect apoptosis indicators.Proteomics was used to analyse differential proteins(DEPs).STEP receptor inhibitor was injected into the lateral ventricles,the rats were administered for seven days,then the apoptosis indicators were detected.Re-sults Salidroside combined with rosavin could reduce neurological function scores in MCAO rats and inhibit cell apoptosis.Quantitative proteomics identified 496 DEPs in brain tissue and discovered core proteins STEP,p38,and CRTC1.Salidroside combined with rosavin could promote the STEP and CRTC1 while in-hibiting p38 protein.After treatment with STEP inhibi-tor,those effects were reversed.Conclusion Salidro-side combined with rosavin can inhibit cell apoptosis in MCAO rats,which is closely related to the regulation of the STEP/p38/CRTC1 signaling pathway.
