Effect of hyperbaric oxygen coupled with cisplatin on the apoptosis of Fadu cells in vitro
10.3760/cma.j.issn.1009-6906.2016.02.009
- VernacularTitle:高压氧联合顺铂对体外培养人下咽癌Fadu细胞凋亡的影响
- Author:
Kai LIU
1
;
Peisong WANG
;
Jine WAN
;
Jian GU
;
Jing XU
;
Zhenju XU
;
Wei LI
Author Information
1. 青岛大学医学院附属医院耳鼻咽喉头颈外科, 山东省青岛,266000
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Hypopharyngeal neoplasm;
Cisplatin;
ATP;
Apoptosis
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2016;23(2):116-119
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hyperbaric oxygen (HBO) coupled with cisplatin on the apoptosis of human hypopharyngeal cancer Fadu cells in vitro and also to observe the effect of HBO exposure at different time points on the efficacy of cisplatin.Methods Human hypopharyngeal cancer Fadu cells for culture were bought from the Tumor Research Institute,Shanghai Jiaotong University.MTT assay was applied to observe the effect of different concentrations of cisplatin on the proliferation inhibition of Fadu cells in vitro.In accordance with different treatment,Fadu cells were divided into 6 groups:group C (the control group),group HBO (the HBO group),group CHT (the chemotherapy group),group HBO + CHT (the HBO + chemotherapy group),group CHT + HBO (the chemotherapy + HBO group and group IC (the HBO immediately combined with chemotherapy group).Cells of various groups were detected by flow cytometry 48 hours after experiment to observe changes in apoptosis,and adenosine triphosphate (ATP) changes in the cells of various groups were measured by ATP kit.Results Apoptosis rates of group C,HBO,CHT,HBO + CHT,CT + HBO and IC were respectively(2.00 ±0.17)％,(2.07 ±0.22)％,(8.73 ±0.74)％,(11.9 ±0.68)％,(9.92 ±0.27)％ and(10.4 ±0.72)％.No statistical significance could be noticed in the apoptosis rates of group C and HBO(P ＞0.05).The apoptosis rates of group CHT,HBO + CHT,CHT + HBO and IC were all higher that those of group C and HBO,and statistical significance could be noted when comparisons were made between them(P ＜0.01).Experiments showed that the apoptosis rate of group HBO + CHT was higher than those of the other groups,with statistical significance(P ＜ 0.05).Suppose the ATP level of group C was 100％,the ATP levels of other groups were respectively 98.7％,83.9％,61.3％,78.5％ and 77.1％.There was statistical significance in the ATP levels of group C and HBO(P ＞0.05).However,the ATP levels of group CHT,HBO + CHT,CHT + HBO and IC were all lower than those of group C and HBO,also with statistical significance (P ＜0.01).The ATP level of group HBO + CHT was significantly lower than those of the other groups,also with statistical significance(P ＜ 0.05).Conclusions Single HBO exposure had no effect on the apoptosis of hypopharyngeal cancer Fadu cells.HBO coupled with cisplatin could enhance the therapeutic efficacy of cisplatin,promote apoptosis,and the efficacy might be associated with the time points of the combined treatment of HBO.
