Effects of acute inhalation of fire extinguishing agent Novec 1230 on blood biochemical indexes in mice
10.3760/cma.j.issn.1009-6906.2015.03.015
- VernacularTitle:Novec 1230灭火剂急性吸入对小鼠血液生化指标的影响
- Author:
Xinhong XU
1
;
Linjun XU
1
;
Lu JIANG
1
;
Qian CHEN
1
Author Information
1. 海军医学研究所, 上海,200433
- Publication Type:Journal Article
- Keywords:
Fire extinguishing agent novec 1230;
Acute inhalation;
Toxicity;
Routine blood indexes;
Blood biochemical indexes
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2015;(3):222-225
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of acute inhalation of fire extinguishing agent Novec 1230 on routine blood indexes and biochemical indexes in mice .Methods One hundred and twenty male ICR mice with an age range of 5 weeks and body mass of 18-22 g were randomly divided into the normal control group and the acute inhalation group , which were further divided into 3 subgroups , each consisting of 30 animals.The animals were exposed to the fire extinguishing agent Novec 1230 at concentration levels of 5%, 10%and 15%for 2 hours respectively , while the animals in the control group were exposed to common room air.Two time points ( at 2 hours and 14 days after inhalation exposure ) were set for each subgroups , which consisted of 15 mice.The routine blood levels and serum biochemical indexes of the mice were detected at the above 2 time points.Results Following exposure to the agent at levels of 5%, 10%and 15%for 2 hours, red blood cell (RBC) counts [(9.99 ±1.16) ×1012,(9.90 ±0.68) ×1012,(8.84 ±1.39) ×1012/L] decreased gradually with the increase in exposure concentrations , and statistical significance could be noted , when they were compared with those of the control group [(9.16 ±1.18)1012/L] (P<0.05).White blood cell (WBC) counts [(4.11 ±0.75) ×109,(6.23 ±1.05) ×109,(6.51 ±1.30) ×109/L] increased gradually with the increase in exposure concentrations , and statistical significance could be seen , when they were compared with those of the control group [(5.38 ±1.46) ×109/L] (P <0.05 or P <0.01).There were changes in hematocrit (HCT), mean corpuscular volume (MCV), platelet counts and hemoglobin (HGB), as compared with those of the control group , but without statistical significance (P<0.05).The levels of alkaline phosphate (ALP) [(218.67 ±0.85),(210.13 ±0.76)and(196.07 ±6.63)U/L] decreased gradually with the increase of exposure levels.The levels of alanine aminotransferase (ALT) were respectively [(51.50 ±1.40), (61.47 ±0.76) and (62.33 ±11.16)U/L], the levels of aspartate aminotransferase (AST) were respectively [(195.03 ±1.60)、(201.30 ±6.56)、(259.43 ±11.82)U/L] , and the levels of creatine kinase (CK) were respectively [(515.50 ±42.95),(834.60 ±76.20) and(915.63 ±24.18)U/L], which were increased with increase in exposure levels .Statistical significance could be observed , when they were compared with those of the control group [(184.97 ±2.46),(39.37 ±0.97),(187.70 ±2.51) and(452.20 ±93.01)U/L] (P<0. 05 or P<0.01).However,the differences in routine blood indexes and serum biochemical indexes were not statistically significant , when comparisons were made between the acute inhalation groups and the control group at day 14 after exposure(P>0.05).Conclusions Acute exposure of fire extinguishing agent Novec 1230 seemed to induce infectious damage , myocardial and heptal damage to mice , however , the damage afflicted on the animals seemed to recover 14 days after exposure .
