From stretching to signal:the sensory roles of YAP1 and PIEZO2 in bladder urothelial cells
10.3969/j.issn.1009-8291.2025.07.013
- VernacularTitle:从拉伸到信号:YAP1和PIEZO2在膀胱尿路上皮细胞感知中的作用
- Author:
Yongxiang SHAO
1
;
Meng CHENG
;
Mengyuan LIU
;
Liangliang XING
;
Zudu FAN
;
Conglei HU
;
Liping YAO
;
Qian ZHANG
;
Fei LIU
Author Information
1. 空军军医大学西京医院泌尿外科,陕西西安 710032;中国人民解放军联勤保障部队第942医院泌尿外科,宁夏银川 750003
- Publication Type:Journal Article
- Keywords:
bladder urothelial cells;
mechanical signal transduction;
piezo type mechanosensitive ion channel component;
Yes-associated protein 1;
connective tissue growth factor
- From:
Journal of Modern Urology
2025;30(7):615-620
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the roles of the mechanoreceptor Yes-associated protein 1(YAP1)and piezo type mechanosensitive ion channel component 2(PIEZO2)in mechanotransduction in mouse bladder urothelial cells.Methods Mouse bladder urothelial cells were subjected to mechanical stretching using the FX-6000T cell stretching system and treated with the YAP1-specific inhibitor verteporfin(VP).The expressions of PIEZO2,YAP1 and connective tissue growth factor(CTGF)at the mRNA and protein levels,as well as changes in cellular adenosine triphosphatase(ATP)concentration,were detected using reverse transcription quantitative PCR(RT-qPCR)and Western blotting(WB).Results After stretching stimulation,under the fluorescence microscope,it was observed that the diameter length of the stretched cells were longer than that before stretching,and the difference was statistically significant(P＜0.05).The expressions of YAP1,PIEZO2 and CTGF at the mRNA and protein levels were increased in the stretched group compared to those of the non-stretched group(P＜0.05).VP effectively reduced the expressions of YAP1,PIEZO2 and CTGF at the mRNA and protein levels after stretching stimulation(P＜0.05).Stretching stimulation significantly increased the intracellular ATP concentration,while VP was able to inhibit the increase in ATP concentration,with a statistically significant difference(P＜0.000 1).Conclusion Stretching stimulation increased the expressions of YAP1 and PIEZO2 in bladder urothelial cells and promoted the release of ATP;verteporfin inhibited the increase in YAP1 activity and the overexpression of PIEZO2 caused by stretching,thereby reducing the release of ATP.It is suggested that mouse bladder urothelial cells may primarily sense mechanical signals through the YAP1-PIEZO2-ATP pathway.
