Effect of hyperbaric oxygen on atherosclerosis in apoE-knockout mice
10.3760/cma.j.issn.1009-6906.2015.03.007
- VernacularTitle:高压氧对载脂蛋白 E 基因敲除小鼠动脉粥样硬化的影响
- Author:
Huilong SHAO
1
;
Qingbao YANG
;
Zhenzhen FAN
;
Ping ZHONG
;
Youcun WANG
Author Information
1. 济南军区总医院康复医学科, 济南,250031
- Publication Type:Journal Article
- Keywords:
Atherosclerosis;
Hyperbaric oxygen;
Apolipoprotein E;
Gene knock-out;
C-reactive protein
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2015;(3):192-195
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of hyperbaric oxygen ( HBO) on the atherosclerosis ( AS) and the possible mechanism in apolipoprotein E ( apoE )-knockout mice by observing the variation of atherosclerotic plaque pathological morphology , blood lipid and related inflammatory cytokines .Methods Twenty-eight 8-week old apoE-knockout male mice were fed with western-diet for 8 weeks.Four mice were randomly sacrificed to collect aortas for Hematoxylin and Eosin ( HE ) staining , and the AS model was ascertained under microscopy .Then, the remaining 24 mice were randomly assigned into the ( HBO) group (n=12) and the normal control group (n=12).The animals in the HBO group were treated with HBO once a day for 30 days and the animals in the control group were left there without HBO treatment .The body mass of the mice were weighed both before and after termination of HBO treatment .The day after termination of HBO treatment, the mice were sacrificed for the detection of blood lipids and serum C-reactive protein (CRP). Histopathological features of aorta were defined by Hematoxylin and Eosin (HE) staining.Subsequently, the area ratio of plaque and lumen and the ratio of intimal and media areas were analyzed by the image processing software.Results Following HBO treatment, there were no significant differences in blood lipid levels , when comparisons were made between the HBO group and the control group (P>0.05), however, the level of serum CRP in the HBO group was significantly lower than that of the control group (P<0.05).The HE staining indicated that large quantities of atherosclerotic plaques in the aorta of the control group and intima were thickened , and the area ratio of plaque and lumen and the ratio of intimal area and media area was obviously larger than those of the animals in the HBO group ( P <0.05).Conclusions HBO could inhibit the development of atherosclerosis , which was not related to the modulation of blood lipid .The effect of HBO on anti-inflammation might be related to further progress of atherosclerosis .
