Analysis of the nucleic acid detection results for six non-influenza viruses in influenza-like illness cases in Shandong Province from 2020 to 2021
10.3760/cma.j.cn112150-20240403-00272
- VernacularTitle:2020—2021年山东省流感样病例6种非流感病毒的核酸检测结果分析
- Author:
Yujie HE
1
;
Zhong LI
;
Julong WU
;
Lin SUN
;
Shaoxia SONG
;
Shu ZHANG
;
Xiaolin LIU
;
Yang DONG
;
Xianjun WANG
;
Zengqiang KOU
;
Ti LIU
Author Information
1. 山东省疾病预防控制中心传染病防制所 山东省预防医学研究院 山东省传染病智慧化监测预警和防控重点实验室（筹），济南 250014
- Publication Type:Journal Article
- Keywords:
Influenza, human;
Communicable diseases;
Multi-pathogen surveillance
- From:
Chinese Journal of Preventive Medicine
2025;59(2):216-221
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the respiratory virus infection status and epidemiological characteristics of influenza-like illness (ILI) cases in Shandong Province during the 2020 -2021 influenza surveillance year. Methods:According to the National Influenza Surveillance Plan (2017 version), throat swab samples of ILI cases were collected from 14 surveillance sentinel hospitals in Shandong Province. Nucleic acid was extracted from all samples. Real-time fluorescence quantitative PCR (RT-PCR) was utilized to detect six common viruses, including human metapneumovirus (HMPV), human parainfluenza virus (HPIV) types 1, 2 and 3, respiratory syncytial virus (RSV), and adenovirus (ADV). Subsequently, the obtained detection results were analyzed.Results:A total of 2 386 specimens were collected, with a detection rate of 24.22% (578). Six viruses were detected, with detection rates of 6.75% (162 cases) for HMPV, 5.87% (140 cases) for RSV, 3.56% (85 cases) for HPIV3, 3.14% (75 cases) for HPIV2, 2.98% (71 cases) for HPIV1, and 2.77% (66 cases) for ADV. There was no significant difference in detection rates between genders, but a notable variation among different age groups ( P<0.001). The highest detection rate was observed in individuals aged 0-4 years (31.94%), followed by those aged≥60 years (26.06%). The prevalence of six viruses showed a monthly variation, with the detection rate of HMPV being higher in December and HPIV1 being higher in February. HPIV2, HPIV3, RSV, and ADV had higher detection rates in November. The co-detection rate of multiple viruses was 0.80%, with RSV being the most common pathogen involved in co-detection, primarily in individuals aged 0-4 years. Conclusion:The detection of six multiple pathogens in ILI cases in Shandong Province is dominated by HMPV, RSV and HPIV3. The prevalence of respiratory viruses varies by age and time.
