NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line BEAS-2B
10.16352/j.issn.1001-6325.2025.08.1010
- VernacularTitle:NF-κB抑制剂或NAC减轻胺碘酮诱导的人肺上皮细胞系BEAS-2B损伤
- Author:
Zezhao JI
1
;
Qi ZHANG
1
;
ABUDUXUKUER·ABULIMITI
1
Author Information
1. 新疆医科大学基础医学院组织与胚胎学教研室,新疆乌鲁木齐 830000
- Publication Type:Journal Article
- Keywords:
amiodarone;
reactive oxygen species;
cell injury;
NF-κB
- From:
Basic & Clinical Medicine
2025;45(8):1010-1015
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of reactive oxygen species(ROS)and pyroptosis in amioda-rone-induced lung epithelial cell injury.Methods The viability of cells treated with amiodarone,amiodarone+N-acetylcysteine(ROS inhibitor,NAC)and amiodarone+NF-κB inhibitors was detected by MTT assay.ROS level was detected by DCFA probe and the levels of IL-6,IL-18,IL-1β and TNF-α in the supernatant were detected by ELISA;NLRP3.Caspase-1 p20 and GSDMD-N protein were detected by Western blot.Results Amiodarone con-centration-dependently reduced cell viability(P＜0.05),NAC and NF-κB attenuated the cytotoxicity of amiodarone(P＜0.05).Amiodarone increased levels of reactive oxygen species,inflammatory factors and pyroptosis-related proteins in lung epithelial cells(P＜0.05),while NAC and NF-κB inhibitors reduced the levels of these indicators(P＜0.05).Conclusions NF-κB inhibitor or NAC attenuates amiodarone-induced injury of human lung epithelial cell line in vitro.
