Exploring the immuno-inflammatory regulatory effects of the Qing Gan San Jie Xiao Ying Formula(QGSJXYF)on Hashimoto's thyroiditis based on thyroid cell-derived exosomes
10.3969/j.issn.1009-9905.2025.04.001
- VernacularTitle:基于甲状腺外泌体探讨清肝散结消瘿方对桥本甲状腺炎免疫炎症的调控作用
- Author:
Si-miao YAO
1
;
Shuo QI
;
Xiao-ning SUN
;
Xin-ai LI
;
Hui-long LI
;
Zhi-guo DING
Author Information
1. 北京中医药大学东直门医院 甲状腺病科(北京 100700);北京中医药大学孙思邈医院 甲状腺病院(陕西 铜川 727100)
- Publication Type:Journal Article
- Keywords:
Hashimoto's thyroiditis;
Qing Gan San Jie Xiao Ying Formula(QGSJXYF);
Exosomes;
Lymphocytes;
Im-mune inflammation
- From:
Chinese Journal of Current Advances in General Surgery
2025;28(4):253-258
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the immuno-inflammatory regulatory effects of the Qing Gan San Jie Xiao Ying Formula(QGSJXYF)on Hashimoto's thyroiditis(HT)by modulating thyroid cell-derived exosomes to provide experi-mental evidence for its immunomodulatory mechanisms.Methods:Nthy-ori-3-1 thyroid cells were treated with QGSJXYF-medicated serum,with untreated cells serving as controls.Exosomes from both groups were extracted and analyzed using nanoparticle tracking analysis(NTA),transmission electron microscopy(TEM),and Western blot to assess concentration,size distribution,morphology,and the expression of characteristic exosomal markers.An inflammatory model of human T lymphocytes(H9)was established and co-incubated with normal exosomes(EXO-C group)or QGSJXYF-treated exosomes(EXO-T group).The levels of inflammatory cytokines in H9 cells were measured using Western blot(WB)and ELISA.Results:Exosome characterization showed that the particle concentration of Nthy-ori-3-1 cell-derived exosomes in both the control and QGSJXYF groups ranged from 1×109 to 1×1011/mL,with particle diameters between 80～300 nm.The exosomes exhibited a typical spherical or cup-shaped morphology with positive expression of TSG101,CD63,and HSP70.Compared with the inflammation model group and the EXO-C group,the EXO-T group significantly reduced the intracellular expression of IL-17A protein in H9 cells(P<0.05)and suppressed IL-17 and IL-6 levels in the cell supernatant(P<0.01).Conclusion:QGSJXYF may exert its anti-inflammatory and thyroid-protective effects by modulating the functional state of thyroid cell-derived exosomes,regulating the inflamma-tory microenvironment,and inhibiting the expression of inflammatory cytokines associated with Hashimoto's thyroiditis.
