Preparation of the Fusion Protein Between Diphtheria Toxin Mutants and the Receptor Binding Domain of Botulinum Neurotoxin Serotype E(EHc)Molecules and the Immunological Effect Evaluation
10.13865/j.cnki.cjbmb.2025.09.1262
- VernacularTitle:白喉毒素突变体与E型肉毒毒素受体结合区融合分子制备和免疫效应评价
- Author:
Qiu-Ju JIA
1
;
Yao-Hui ZHAO
;
Xiao-Yu LIU
;
Shuo YU
;
Jian-Sheng LU
;
Yun-Zhou YU
;
Ming LIAO
Author Information
1. 广西医科大学基础医学院,南宁 530000;军事科学院军事医学研究院,北京 100850
- Publication Type:Journal Article
- Keywords:
diphtheria toxin(DT);
cross-reacting material 197(CRM197)mutant;
fusion protein;
sub-unit vaccine;
immunological effect
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(10):1421-1431
- CountryChina
- Language:Chinese
-
Abstract:
CRM 197(cross-reacting material 197),a naturally occurring mutant of diphtheria toxin,is a safe and effective vaccine vector and extensively used on developing conjugate or combined vaccines.The mutant loses its enzymatic activity,but fully retains its receptor-binding ability and immunogenicity.In current work,the diphtheria toxin mutant CRM 197 and its fusion proteins with the receptor-binding do-main of botulinum neurotoxin serotype E(EHc)were developed using genetic engineering technology.These recombinant proteins were confirmed by Western blotting and SDS-PAGE.BALB/c mice were im-munized with the CRM197-EHc and EHc-CRM197 fusion proteins,and their immunogenicity was evalua-ted.These two fusion protein molecules,CRM197-EHc and EHc-CRM197,as subunit vaccines,elicited a robust humoral immune response targeting both CRM197 and EHc antigens in the immunized mice.Compared to the mixture of CRM197 and EHc,the mice vaccinated with the fusion proteins(CRM197-EHc and EHc-CRM197)induced higher levels of anti-CRM197 antibodies,and the mice vaccinated with EHc-CRM197 also generated strongest anti-EHc antibodies.Consequently,as a carrier molecule in the fusion protein vaccine,EHc enhances the immunogenicity of CRM197 molecules.Likewise,CRM197 boosts the immunogenicity of EHc in the EHc-CRM197 fusion protein.
