Pathogenicity and multiple detection methods for infectious diarrhea in children under 5 years old in Zhongshan City from 2023 to 2024
10.12138/j.issn.1671-9638.20252963
- VernacularTitle:2023-2024年中山市5岁以下儿童感染性腹泻病原学及多重检测方法评估
- Author:
Wuyang SHI
1
;
Ting OUYANG
;
Shuhuan YANG
;
Yunxia MAO
;
Yanheng WU
;
Bo HE
Author Information
1. 中山市疾病预防控制中心病原微生物与生物检验所,广东 中山 528400
- Publication Type:Journal Article
- Keywords:
infectious diarrhea;
pathogen spectrum;
multiple gene detection;
consistency evaluation
- From:
Chinese Journal of Infection Control
2025;24(10):1402-1408
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the pathogen spectrum characteristics of infectious diarrhea in children under 5 years old in Zhongshan City from 2023 to 2024,and evaluate the application value of multiple detection technique in monitoring diarrhea syndrome.Methods Diarrhea specimens from hospitalized children under 5 years old in 4 senti-nel hospitals in Zhongshan City from 2023 to 2024 were collected,Luminex? multi-pathogen detection kit(GPP)was used for screening 16 types of pathogens,and fluorescence quantitative polymerase chain reaction(qPCR)was simultaneously used to verify the consistency of detection results of 5 diarrhea virus.Results A total of 578 dia-rrhea specimens were collected,and the positive detection rate of pathogens was 67.13％(n=388).The positive detection rate of viral pathogens was 38.24％,mainly norovirus(21.63％),rotavirus A(10.90％),and sapovirus(4.67％).The positive detection rate of bacterial pathogens was 41.00％,mainly Salmonella spp.(19.55％),Clostridioides di f ficile toxin A/B(14.71％),and Campylobacter sp p.(8.82％).Cryptosporidium,Entamoeba histolytica,and Giardia were not detected.The consistency between GPP and qPCR in detecting viral pathogens reached 95.16％,with a Kappa value of 0.897(x2=465.36,P＜0.001).Conclusion The main pathogens causing diarrhea in children in Zhongshan City are norovirus,Salmonella,and Clostridioides toxin A/B.GPP technique can efficiently construct a multi-pathogen spectrum,and provide reliable technical support for optimizing the monitoring system of diarrhea syndrome.
