Effects of simulated heliox diving on blood cells of the divers at different high altitudes
10.3760/cma.j.issn.1009-6906.2012.06.003
- VernacularTitle:模拟不同海拔高原环境下氦氧潜水对潜水员血细胞的影响
- Author:
Hui-jun HU
1
;
Dan-feng FAN
1
;
Chen YANG
1
;
Ling ZHAO
1
;
Ru-gang ZHAO
1
;
Xiao-wen PAN
1
Author Information
1. 海军总医院全军高压氧治疗中心,北京,100048
- Publication Type:Journal Article
- Keywords:
High altitude;
Heliox diving;
Blood cell;
T lymphocyte
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2012;19(6):327-330
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of simulated heliox diving on blood cells of the divers at different high altitudes.Methods In the dual-purpose hyperbaric and hypobaric chamber complex,4 divers conducted a series of simulated dives for a succession of 9 days at various altitudes and under different simulated environmental conditions:the 30 m dive at an altitude of 3000 m,the 30 m dive at an altitude of 4000 m,and the 30 m and 50 m dives at an altitude of 5200 m.Blood samples were taken respectively at the following time points:on the morning of the first day of the experiment before they entered the chamber on an empty stomach;on the morning of the third day of the experiment at an altitude of 3000 m also on an empty stomach; at 17:30 pm before supper on the fourth day of the experiment,when the divers were decompressed and returned to the surface at an altitude of 3000 m,upon completion of the 30 m-dive; at 17:30 pm before supper on the sixth day,when the divers returned to the surface at an altitude of 4000 m,upon completion of the 30 m-dive; on the morning of the seventh day on an empty stomach,the divers stayed overnight at an altitude of 5200 m; at 23:00 on the eighth day,when the divers were decompressed to an altitude of 5200 m,upon completion of the 50 m-dive; before lunch on the ninth day,when the divers came out of the chamber upon completion of the experiment.Blood components including WBC,WBC classification,RBC,hemoglobin,lymphocyte,granulocyte,hemoglobin,hematocrit,platelet and T lymphocyte subpopulation,were measured with flow cytometry.Results White blood cells detected at various time points both before and after the experiment tended to increase gradually,but without statistical significance (P ＞ 0.05).When the divers stayed at the height of 5200 m above sea level,the percentage of granulocyte was significantly higher [(6.21 ± 3.27) × 109/L] than those at the sea level [(3.22 ± 1.18) × 109/L] and at 3000 m above sea level [(3.43 ± 1.18) × 109/L] (P ＜ 0.01),while the percentage of lymphocyte decreased significantly (P ＜ 0.01) and corresponding changes in the amount of granulocyte and lymphocyte could be observed at the same time points (P ＜ 0.05).When the divers were decompressed to the altitude of 5200 m upon completion of the 50 m simulated dive at 5200 m above sea level,lymphocyte counts significantly increased [(2.28 ± 0.30) × 109/L],when compared with those at the plain [(2.02 ±0.15) × 109/L] and at 3000 m[(2.20 ±0.26) × 109/L] (P ＜ 0.05).No obvious changes could be noted in white cells at other time points.No statistical significance could be noticed in changes of red blood cell,hemoglobin,hematocrit,platelet and T lymphocyte subpopulation at various time points during the experiment,when compared with those at the plain and at 3000 m (P ＞ 0.05).Conclusions The simulated heliox diving at different high altitudes didnt seemed to have obvious effects on the blood routines of the divers.
