Enhancement of HIV-1 replication by CD4+T lymphocytes in peripheral blood of syphilis patients and its potential molecular mechanisms
10.12138/j.issn.1671-9638.20256619
- VernacularTitle:梅毒患者外周血CD4+T淋巴细胞增强HIV-1复制及其潜在的分子机制
- Author:
Chun ZHANG
1
;
Wujian KE
;
Yinbo JIANG
;
Shixing TANG
Author Information
1. 南方医科大学公共卫生学院流行病学系,广东 广州 510515
- Publication Type:Journal Article
- Keywords:
Treponema pllidum;
HIV-1;
infection;
CXCL5;
CXCL8
- From:
Chinese Journal of Infection Control
2025;24(5):647-656
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of peripheral blood CD4+T lymphocytes of syphilis patients on human immunodeficiency virus type 1(HIV-1)infection and replication,and explore the molecular mechanism of Trepone-ma pallidum(TP)in facilitating HIV-1 replication.Methods Peripheral blood mononuclear cells(PBMCs)were isolated from syphilis patients and TP-uninfected healthy blood donors,then infected with HIV-1 in vitro.HIV-1 replication level was studied.PBMCs of healthy persons were stimulated with TP and then infected with HIV-1.CD4+T lymphocytes were isolated using flow cytometry,and changes in host gene expression profiles were verified using messenger RNA(mRNA)high-throughput sequencing and real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)methods.Results CD4+T lymphocytes from TP-infected persons and TP-stimulated CD4+T lymphocytes from healthy persons could significantly enhance HIV-1 infection and viral replication levels.The HIV-1 p24 content in the supernatant of cultured cells and intracellular HIV-1 DNA copy number were both higher than those in the control group(both P＜0.05).There were significant differences in the mRNA gene ex-pression profiles between the TP stimulation combined with HIV-1 infection group and the HIV-1 infection alone group.Among them,the expression levels of chemokines CXCL5 and CXCL8 were significantly upregulated in the TP stimulation combined with HIV-1 infection group,indicating that chemokines might play an important role in the enhancement of HIV-1 infection and replication by TP.Conclusion TP infection can significantly enhance the sus-ceptibility of peripheral blood CD4+T lymphocytes to HIV-1 and promote virus replication,providing new scientific evidences for TP infection promoting HIV-1 infection.
