Protective effects of adenosine preconditioning on intestinal mucosa in rats with open abdominal injury coupled with seawater immersion
10.3760/cma.j.issn.1009-6906.2017.03.002
- VernacularTitle:腺苷预处理对腹部开放伤海水浸泡大鼠肠道黏膜的保护作用
- Author:
Jiang PU
1
;
Shaoxin WANG
1
;
Xiaohui WANG
1
;
Lan YU
1
;
Zhe LUO
1
;
Zeping HUANG
1
;
Lihong CUI
1
Author Information
1. 海军总医院消化内科, 北京,100048
- Publication Type:Journal Article
- Keywords:
Adenosine preconditioning;
Abdominal open injury;
Seawater immersion;
Intestinal mucosa;
Apoptosis
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2017;24(3):173-176,195
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of adenosine preconditioning on the intestinal inflammatory response,the damaged mucosal tissue and apoptosis of intestinal epithelial cells in rats with abdominal open injury coupled with seawater immersion.Methods Sixty male Wistar rats with abdominal open injury were randomly divided into 3 groups,each consisting of 20 animals.The animals in group A had 0.9％ sodium chloride solution immersion + caudal vein injection of sterilization water (3 mg/kg).The animals in group B received seawater immersion + caudal vein injection of sterile water (3 mg/kg) and the animals in group C were given seawater immersion + caudal vein injection of adenosine pretreatment (3 mg/kg).All animals were sacrificed 6 hours after immersion.Pathological changes in intestinal mucosa were closely observed by histopathology.Levels of TNF-α and IL-6 were detected by enzyme-linked immunosorbent assay (ELISA).Caspase-3 protein was detected by immunohistochemistry.Apoptosis of the cells in intestinal mucosa were monitored by TUNEL method.Results The intestinal pathological scores of TNF-α and IL-6 for the animals in group B(3.98 ±0.41)were significantly higher than those in group A (2.46 ±0.39) (P ＜0.01).The TNF-α and IL-6 levels for the animals in group B [(2.38 ±0.39) μg/L and(883.11 ±34.22) ng/L] were significantly increased,as compared with those in group A [(1.01 ± 0.06) μg/L and (258.09 ± 6.52) ng/L] (P ＜ 0.01).The TNF-α and IL-6 levels for the animals in group C[(1.88 ± 0.21)pg/L and (582.13 ±19.44)ng/L] were significantly decreased,as compared with those in group B (P ＜ 0.01).The level of Caspase-3 and percentage of apoptotic cells in the intestinal mucosa of the animals in group B [(27.9 ± 4.3) ％ and (24.5 ± 3.1) ％]were significantly higher than those of the animals in group A [(13.8 ± 2.6) ％ and (16.4 ± 2.3) ％] (P ＜0.01),and the expression level of Caspase-3 and percentage of apoptotic cells of the animals in group C [(20.2 ± 3.4) ％ and (19.9 ± 2.9) ％] were significantly lower than those of the animals in group B (P ＜ 0.01).Conclusions Adenosine pretreatment could significantly improve intestinal mucosal inflammatory response in seawater immersion rats coupled with open abdominal open injury,alleviate the destruction of intestinal epithelial structure,and reduce apoptosis of intestinal mucosal epithelial cells.
