Effects of different transplantation routes and fecal status on structural abundance of gut microbiota
10.3969/j.issn.1005-4847.2024.12.004
- VernacularTitle:不同移植途径及粪便状态对肠道菌群结构丰度的影响
- Author:
Kaihui LIU
1
;
Yaxi GUO
1
;
Xiaopeng DU
1
;
Zhaohua WANG
1
;
Hua ZHU
1
Author Information
1. 中国医学科学院医学实验动物研究所,国家动物模型技术创新中心,国家卫生健康委员会人类疾病比较医学重点实验室,国家人类疾病动物模型资源库,北京 100021
- Publication Type:Journal Article
- Keywords:
intestinal flora;
human flora-associated model;
16 S rRNA sequencing;
flora structure
- From:
Acta Laboratorium Animalis Scientia Sinica
2024;32(12):1532-1542
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of fecal status and transplantation method on the intestinal flora structure after fecal microbiota transplantation in germ-free mice.Methods Thirty-six C57BL/6 mice were divided randomly into three groups:fresh fecal gavage transplantation(group A),frozen fecal gavage transplantation(group B),and frozen fecal rectal transplantation groups(group C).Feces were collected at 2,4 and 6 weeks after transplantation.All mice were sacrificed at 6 weeks to obtain the contents of the small and large intestines.The structure and function of the gut microbiota and dynamic trends in microbial changes were analyzed by 16 S rRNA sequencing.Results The diversity of the small intestine microbes in both the group A and group C were similar to those in the group B,according to α-diversity analysis(P＞0.05),but the diversity of large intestine microbes was significantly increased(P＜0.001).According toβ-diversity analysis,small intestine samples from the group A and group B clustered in the same area,indicating that the microbial community compositions were similar(P＞0.05),but samples from the large intestine were distributed in different areas,showing significant differences(P＜0.001).Small and large intestine samples from the group B and group C were distributed in different areas,with significant differences(P＜0.001).Linear discriminant analysis effect size showed that Bacteroidota were relatively dominant in the group A,while Verrucomicrobiota and Proteobacteria were relatively dominant in the group B and Firmicutes were relatively dominant in the group C.Functional prediction using PICRUSt2 software showed that neither fecal status nor the method of transplantation affected the functions of the microbial community.Conclusions Both fresh fecal gavage and frozen fecal rectal transplantation can enhance the microbial diversity of the large intestine,compared with frozen fecal gavage transplantation.Fecal status does not affect the gut function and colonization trends of the microbiota,whereas the method of transplantation affects the colonization trends but not the functions of the microbiota.
