The impact of FOXP2 mediated by the Hedgehog signaling pathway on glycolysis participating in the proliferation and metastasis in uterine leiomyoma cells
10.3969/j.issn.1006-7795.2025.01.018
- VernacularTitle:Hedgehog信号通路介导下FOXP2对糖酵解参与子宫肌瘤细胞增殖和转移的影响
- Author:
Yan YANG
1
;
Ni TIAN
;
Ling LONG
;
Zhenxiang JIA
Author Information
1. 贵州省职工医院(贵州省人民医院花溪分院贵州大学附属第二医院)妇科,贵阳 550000
- Publication Type:Journal Article
- Keywords:
forkhead box protein p2;
Hedgehog signal pathway;
glycolysis;
uterine fibroids
- From:
Journal of Capital Medical University
2025;46(1):115-124
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of forkhead box protein p2(FOXP2)in the proliferation and metastasis of uterine fibroid cells by regulating glycolysis mediated by Hedgehog signaling pathway.Methods Fresh hysteromyoma tissue was obtained from 10 patients who underwent myomectomy in the Department of Gynecology of Guizhou Provincial Hospital of Workers,and hysteromyoma cells were digested and separated.Cells were transfected with lentivirus shRNA targeting FOXP2(sh-FOXP2),negative control(sh-NC)and FOXP2 overexpression vectors(oe-FOXP2),and negative control(oe-NC).Colony formation test and Transwell assay were used to evaluate the cell proliferation and metastasis ability.Glucose uptake,lactic acid production and extracellular acidification rate(ECAR)were measured to detect the aerobic glycolysis ability of cells.A xenograft tumor model was established by up-regulating uterine leiomyoma cells with FOXP2 to explore the regulatory effect of FOXP2 in vivo.Results Compared with oe-NC group,the cell viability,colony number,migrating cell number,glucose uptake,lactic acid production and ECAR in oe-FOXP2 group decreased significantly(P＜0.05).Compared with sh-NC group,the cell viability,colony number,migrating cell number,glucose uptake,lactic acid production and ECAR of uterine fibroids in sh-FOXP2#1 and sh-FOXP2#2 groups increased significantly(P＜0.05).Compared with oe-NC+vector group,the number of colonies,migrating cells,glucose uptake,lactic acid production,ECAR,Shh and Gli proteins in oe-FOXP2+vector group decreased significantly(P＜0.05).Compared with oe-FOXP2+vector group,the number of colonies,migrating cells,glucose uptake,lactic acid production,ECAR,Shh and Gli proteins in oe-FOXP2+SAG group were significantly increased(P＜0.05).Compared with oe-NC group,the tumor weight,the proportion of Ki-67 positive cells and the proteins of Shh,Gli,GLUT1 and PGAM1 in oe-FOXP2 group decreased significantly(P＜0.05).Compared with oe-FOXP2 group,the tumor weight,the proportion of Ki-67 positive cells and the proteins of Shh,Gli,GLUT1 and PGAM1 in oe-FOXP2+SAG group increased significantly(P＜0.05).Conclusions FOXP2 participates in the proliferation and metastasis of uterine leiomyoma cells by regulating glycolysis mediated by Hedgehog signaling pathway.
