Network Pharmacology Study of Tongguanteng Injection Inhibits the Proliferation and Migration in Cervical Cancer Cells via Targeting FGF2/MAPK
10.11842/wst.20240414003
- VernacularTitle:通关藤注射液靶向FGF2/MAPK抑制宫颈癌细胞增殖和迁移的网络药理学研究
- Author:
Dongxu ZHU
1
;
Zhaoying CAI
;
Jie XIANG
;
Ruoyu ZHOU
;
Qiong XU
;
Yayun QIAN
;
Hongmei LU
Author Information
1. 扬州大学医学院 扬州 225001
- Publication Type:Journal Article
- Keywords:
Tongguanteng injection;
Cervical cancer;
Cell proliferation;
Cell migration;
Network pharmacology
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2025;27(4):1179-1187
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the targets and mechanisms of Tongguanteng Injection in inhibiting the proliferation and migration of cervical cancer.Methods The biological activity of Tongguanteng Injection in inhibiting human cervical cancer SiHa cells was determined by MTT method.Detecting the effect of Tongguanteng Injection on SiHa cell migration through wound healing assay.Using network pharmacology to collect the key targets for treating cervical cancer,and perform molecular docking and enrichment analysis on the targets.Immunohistochemistry and Western blot were used to detect the key proteins to validate the network pharmacology predictions.Result Tongguanteng Injection significantly inhibited the proliferation and migration in a dose-dependent manner in human cervical cancer SiHa cells.Based on the main active ingredients of Marsdenia tenacissima,81 therapeutic targets for cervical cancer were obtained,which may treat cervical cancer by affecting key proteins such as FGF2,MAPK1,and MAPK3.Immunohistochemical results indicated that FGF2,MAPK1 and MAPK3 were expressed in cervical cancer tissues.The western bolt assays showed that Tongguanteng Injection could significantly reduce the FGF2 protein expression.Meanwhile,the MAPK1 and MAPK3 protein expressions were significantly increased.Conclusion Tongguanteng Injection may regulate the FGF2,MAPK1 and MAPK3,effectively impede the proliferation and migration of cervical cancer.
