Effects of hydrogen-rich solution on differentiation state of Schwann cells in mice with diabetic peripheral neuropathy
10.3760/cma.j.cn131073-20240820-00918
- VernacularTitle:富氢液对糖尿病周围神经病变小鼠雪旺细胞分化状态的影响
- Author:
Shu WANG
1
;
Yang JIAO
Author Information
1. 天津市胸科医院体外循环科，天津 300000
- Publication Type:Journal Article
- Keywords:
Diabetic neuropathies;
Hydrogen;
Schwann cells;
Cell dedifferentiation
- From:
Chinese Journal of Anesthesiology
2025;45(9):1179-1184
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of hydrogen-rich solution (HRS) on the differentiation state of Schwann cells in mice with diabetic peripheral neuropathy.Methods:Twenty-four C57BKS background specific-pathogen-free male diabetic (db/db) mice, aged 16 weeks, were divided into 3 groups ( n=8 each) using a random number table method: diabetes mellitus group (group db/db), diabetes mellitus + low-dose (5 ml/kg) HRS group (group db+ LH), and diabetes mellitus + high-dose (10 ml/kg) HRS group (group db+ HH). Eight heterozygous non-diabetic (db/m) mice, littermates of db/db mice, weighing 25-30 g, aged 16 weeks, served as non-diabetic control group (db/m group). Mice in db+ LH and db+ HH groups received an intraperitoneal injection of HRS once daily for 8 consecutive weeks, while mice in db/m and db/db groups received the equal volume of normal saline instead. Immediately after intraperitoneal injection (T 0) and at 2, 4, 6 and 8 weeks after intraperitoneal injection (T 1-4), the blood glucose and body weight were monitored and thermal paw withdrawal latency (TWL) and mechanical paw withdrawal threshold (MWT) were measured. After the behavioral testing, sciatic nerve tissues were collected to determine the expression of p75 neurotrophin receptor (p75NTR), myelin basic protein (MBP), myelin protein zero (MPZ) and sirtuin 1 (SIRT1) (by Western blot) and the expression of p75NTR and SIRT1 in Schwann cells (by immunofluorescence). Results:Compared with db/m group, the body weight and blood glucose concentrations were significantly increased, the TWL was prolonged, and the MWT was increased at each time point, the expression of MBP and MPZ in the sciatic nerve was down-regulated, the expression of SIRT1 in the sciatic nerve and Schwann cells was down-regulated, and the expression of p75NTR in the sciatic nerve and Schwann cells was up-regulated in db/db group, db+ LH group and db+ HH group ( P<0.05). Compared with db/db group, the TWL was significantly shortened at T 1-4, and the MWT was decreased at T 3-4 in db+ LH group, the TWL was significantly shortened, and the MWT was decreased at T 1-4 in db+ HH group, and the expression of MBP and MPZ in the sciatic nerve was significantly up-regulated, the expression of SIRT1 in the sciatic nerve and Schwann cells was up-regulated, the expression of p75NTR in the sciatic nerve and Schwann cells was down-regulated ( P<0.05), and no statistically significant changes in the body weight or blood glucose concentration were found at any time point in db+ LH and db+ HH groups ( P>0.05). Compared with db+ LH group, the MWT was significantly decreased at T 1-2 in db+ HH group ( P<0.05). Conclusions:The mechanism by which HRS alleviates diabetic peripheral neuropathy may be related to up-regulating the expression of SIRT1, thereby reversing the dedifferentiation of Schwann cells in mice.
