Drug resistance detection and molecular typing of diarrheagenic Escherichia coli in patients with diarrhea in Xiangshan County
10.13602/j.cnki.jcls.2025.05.01
- VernacularTitle:象山县腹泻患者致泻性大肠埃希菌的耐药性检测及分子分型
- Author:
Liuyan ZHANG
1
;
Di FANG
;
Shuo YE
;
Xiaozhen ZHU
;
Chuanjing XUE
Author Information
1. 象山县疾病预防控制中心,浙江象山 315700
- Publication Type:Journal Article
- Keywords:
diarrheagenic Escherichia coli;
virulence gene;
drug resistacne;
molecular typing
- From:
Chinese Journal of Clinical Laboratory Science
2025;43(5):321-326
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the epidemiological status and molecular characteristics of diarrheagenic Escherichia coli(DEC)infection in Xiangshan County,providing a basis for its infection surveillance and outbreak control.Methods The real-time fluores-cence quantitative PCR(qRT-PCR)was used to detect virulence genes in isolated E.coli strains.The identified DEC strains were per-formed whole genome sequencing(WGS).The sequencing data were utilized for bacterial resistance gene analysis,multilocus sequence typing(MLST),and core genome multilocus sequence typing(cgMLST).Results A total of 29 DEC strains were isolated,with a detection rate of 9.8%.Enteroaggregative E.coli(EAEC)and enterotoxigenic E.coli(ETEC)were the main strains,accounting for 51.7%and 31.1%,respectively,while enteropathogenic E.coli(EPEC)had a relatively low proportion(17.2%).Drug resistance gene analysis showed that these strains were generally resistant to multiple antibiotics such as fluoroquinolones and carbapenems.MLST typing identified 19 sequence types(STs),including one novel ST type(STcpEC1).Among them,ST117,ST2178,ST31,and ST48 were dominant types.ETEC strains were mainly concentrated in the clonal complex CC-10,while the molecular typing of EAEC and EPEC strains was more dispersed.cgMLST analysis further confirmed the genetic characteristics of different DEC strains.Conclusion DEC infections in Xiangshan County are mainly caused by EAEC,ETEC,and EPEC.These strains have abundant resistance genes.Among them,the molecular typing of EAEC and EPEC is relatively dispersed,while ETEC is concentrated in the clonal complex CC-10.
