Recent Advances in Cell Models of HBV Infection in Vitro
10.3969/j.issn.1671-7414.2025.03.040
- VernacularTitle:HBV感染体外细胞模型的最新研究进展
- Author:
Mengru LIU
1
;
Ningning WANG
;
Qingyang SUN
;
Jun CHENG
;
Yuzhu DAI
Author Information
1. 蚌埠医科大学检验医学院,安徽 蚌埠 233000;中国人民解放军联勤保障部队第九〇三医院检验科,杭州 310013
- Publication Type:Journal Article
- Keywords:
hepatitis B virus;
in vitro cell models;
covalently closed circular DNA;
primary human hepatocytes
- From:
Journal of Modern Laboratory Medicine
2025;40(3):213-220
- CountryChina
- Language:Chinese
-
Abstract:
Hepatitis B virus(HBV)infection is worldwide and poses a serious threat to human health.Existing conventional therapeutic drugs(e.g.,interferon-alpha,nucleoside analogs)can inhibit intrahepatic replication of HBV,but it is difficult to remove HBV covalently closed circular DNA(cccDNA)from the nucleus of hepatocytes,resulting in the virus not being easily cleared and the formation of a chronic infection that is difficult to cure.However,the emergence of targeted drugs is expected to destroy cccDNA making a cure for HBV infection possible.To achieve this,in vitro cell models of HBV infection and replication are needed to screen for targeted drugs.In recent years,in vitro cell models for HBV research have made fundamental progress,providing a platform for a better understanding of virus-host interactions,in vitro studies of the HBV life cycle,and the screening and evaluation of novel antiviral drugs.In this review,the research progress of HBV infection and replication cell models,and the characteristics and limitations of different cell models are reviewed,aiming to provide a basis for exploring the mechanism of complex chronic HBV infection,screening targeted drugs and selecting suitable HBV cell models in vitro.
