Comparative Analysis of Two Chemiluminescent Immunoassay Systems for the Detection of Serum HBsAg Mutants
10.3969/j.issn.1671-7414.2025.03.036
- VernacularTitle:两种化学发光免疫分析系统对血清HBsAg突变株的检测能力比较分析
- Author:
Yong NI
1
;
Dandan CHEN
;
Meina GONG
;
Xiang NI
Author Information
1. 芜湖市中医医院检验科,安徽 芜湖 241000
- Publication Type:Journal Article
- Keywords:
HBsAg;
mutant strain;
CLIA;
qRT-PCR
- From:
Journal of Modern Laboratory Medicine
2025;40(3):195-198,220
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and compare the ability of two chemiluminescent immunoassay systems for detecting HBsAg mutant strains.Methods The two chemiluminescent immunoassay systems,I3000 and Liaison XL,were used to detect nine HBsAg mutant strains with known HBsAg mutation sites respectively,and to compare the detection ability of these two systems for the nine HBsAg mutant strains.The clinical serum samples that were negative for HBsAg by the I3000 system were reviewed by the Liaison XL system for HBsAg,qRT-PCR amplification primers were designed for the HBsAg mutant strains with positive results by the Liaison XL system,and fluorescence quantitative PCR and Hepatitis B serologic tests were performed on the clinical samples that had inconsistent negative-positive results between the two aforementioned systems.Results The Liaison XL detected all nine HBsAg mutant strains with known HBsAg mutation sites with positive results,whereas the I3000 detected six of them with positive but three with negative results.Using the Liaison XL system to test 1 520 samples from hospitalized patients who were negative for HBsAg with the I3000 detection system,four positive results were found,including one positive result by the fluorescence quantitative PCR assay,which belonged to the HBsAg mutant of the mutant strain P124L-F/Y143H-D144E-G145R and the patient's test values for HBeAb and HBcAb were high,suggesting that the patient was a minor triple positive.Conclusion Liaison XL can detect the P124L-F/Y143H-D144E-G145R HBsAg mutant in clinical samples,and it is very important to select HBsAg test reagents with strong HBsAg mutant recognition and capture ability.
