Expression of GLUT1 in Lung Adenocarcinoma Tissues and Its Impact on Cell Cycle and Proliferation
10.3870/j.issn.1672-0741.25.02.012
- VernacularTitle:GLUT1在肺腺癌组织中的表达及其对细胞周期和增殖的影响
- Author:
Huanhuan CHENG
1
;
Yajun WEI
;
Liru LIU
Author Information
1. 河北省肺病重点实验室,石家庄 050001
- Publication Type:Journal Article
- Keywords:
lung adenocarcinoma;
glucose transporter 1;
butyrate metabolism;
proliferation;
cell cycle
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2025;54(5):635-644
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the expression of glucose transporter 1(GLUT1)in lung adenocarcinoma tissues and its effects on cancer cell proliferation,migration,invasion,and cell cycle progression.Methods Prognostic signature genes related to butyrate metabolism in lung adenocarcinoma were screened based on the TCGA-LUAD and GEO databases.Western blot,qPCR,and immunohistochemistry(IHC)were used to detect GLUT1 expression levels.After transfecting A549 and H1299 lung adenocarcinoma cell lines with GLUT1-specific siRNA,CCK-8 assay,colony formation assay,scratch assay,and Transwell assay were performed to investigate the effects of GLUT1 silencing on cell proliferation,clonogenic potential,migration,and invasion,respectively.Flow cytometry was used to analyze cell cycle changes.Results GLUT1 expression was significantly higher in lung adenocarcinoma tissues than normal tissues(P＜0.05).Inhibition of GLUT1 significantly reduced the proliferation,clono-genicity,migration,and invasion abilities of A549 and H1299 cells(P＜0.05).Knockdown of GLUT1 significantly suppressed the expression of CyclinD1 and CDK6,and increased the proportion of cells in the G1 phase(P＜0.05).Conclusion GLUT1 is highly expressed in lung adenocarcinoma tissues and influences patient prognosis by regulating cell cycle progression and pro-liferation,suggesting its potential as a novel therapeutic target for lung adenocarcinoma.
