Protective effect of sipunculusnudus polysaccharide on HUVEC cell damage induced by 137 Cs γ-ray
10.3760/cma.j.issn.1009-6906.2019.02.004
- VernacularTitle:方格星虫多糖对HUVEC细胞137Cs γ射线损伤的保护作用
- Author:
Chunli GAO
1
;
Qingrong WANG
;
Xianrong SHEN
;
BAISHUANG
;
Xiuting HU
;
Ying HE
Author Information
1. 上海海洋大学,上海,201306
- Publication Type:Journal Article
- Keywords:
Sipunculus nudus polysaccharide;
HUVEC cell;
137Cs γ-ray;
Radiation damage;
Cell apoptosis;
Reactive oxygen
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2019;26(2):91-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of sipunculusnudus polysaccharide (SNP) on HUVEC cell damage induced by ionizing radiation.Methods The survival rate of HUVEC cells was detected by CCK-8,after irradiation with 137Cs γ-rays and pre-treatment with SNP,and optimal absorption dose rate of 137Cs γ-ray was 8 Gy and optimal concentration of SNP was confirmed to be 100 g/mL.The radiation sensitivity of HUVEC cells pretreated with SNP was detected by clone formation assay.Apoptosis rate of HUVEC cells was measured by Hoechst33342/PI staining and flow cytometry.Results The results indicated that the survival rate of HUVEC cells decreased significantly with the increase of irradiation dose (P ＜ 0.01).When pretreated with SNP,the survival rate of HUVEC cells significantly increased,and it was positively correlated with the concentration of SNP.When the end concentration of SNP was as high as 100 μg/ml,the survival rate reached the highest (84.96％),and the survival rate after irradiation increased from 63.43％ to 84.96％ (P ＜0.01).The rate of clone formation in the SNP-treated group was also significantly higher as compared with that of the control group(P ＜ 0.01).Compared with the irradiation model group,the apoptosis rate and intracellular reactive oxygen species (ROS) levels in the SNP-treated group decreased significantly,with the apoptosis rate decreasing from 15.6％ to 6.8％,and ROS level decreasing from 47.10 × 104 to 41.77 × 104,with statistical signficance (P ＜ 0.05 or P ＜ 0.01).The blue fluorescence of the Hoechst 33342 coloring and red fluorescence of PI coloring also decreased signficantly in the SNP-treated group (P ＜ 0.01).Conclusion The results suggested that SNP pretreatment could increase cell survival rate and cloning rate after irradiation,reduce radiation-induced apoptosis and intracellular ROS levels effectively,indicating that SNP treatment could exert a certain protective effect on HUVEC cell damage induced by ionizing radiation.
