Effects of hyperbaric oxygen on Th17/Treg cell infiltration in the brain tissue of Alzheimer disease mice
10.3760/cma.j.issn.1009-6906.2019.01.011
- VernacularTitle:高压氧介导的脑内Th17/Treg细胞比例调控对阿尔茨海默病小鼠模型中淀粉样蛋白的作用研究
- Author:
Yinfeng SUN
1
;
Min FU
1
;
Bei TONG
1
;
Jianliang FU
1
Author Information
1. 上海交通大学附属第六人民医院高压氧科,上海,200233
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Alzheimer disease;
Th17 cell;
Treg cell
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2019;26(1):47-52
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of hyperbaric oxygen (HBO) on Th17/Treg cell infiltration in the brain tissue of Alzheimer disease (AD) mice,and also to identify possible mechanism involved for the improvement of AD conditions.Methods Following HBO treatment of APP/PS1 double transgenic mice,immunofluorescence staining and Western blotting were used to detect the deposition of Aβ in the brain tissue.Flow cytometry was used to analyze the infiltration of Th17 cells and Treg cells in the brain tissue.Results The deposition of Aβ in the brain tissue of AD mice after HBO therapy was significantly reduced (P ＜ 0.01).In addition,HBO would increase the expression of IL-17,while it could significantly decrease the expression of FOXP3 (P ＜ 0.01).Flow cytometry detection results indicated that HBO therapy could up-regulate the proportion of Th17/Treg cells in the brain tissue of AD mice (P ＜ 0.01).Conclusion HBO could decrease Aβ deposition in the brain tissue through up-regulation of the proportions of Thl7/Treg cells in the brain tissue of AD mice.
