Determination of the Particle Size of Medium and Long Chain Fat Emulsion Injection(C8～24Ve)by Laser Diffraction Method
10.3870/j.issn.1004-0781.2025.11.009
- VernacularTitle:激光衍射法测定中/长链脂肪乳注射液(C8～24Ve)粒度
- Author:
Qiaoru XUE
1
;
Hua CHEN
;
Aiping OU
;
Hui WU
;
Langui XIE
;
Feng DENG
Author Information
1. 广东省药品检验所,广州 510663
- Publication Type:Journal Article
- Keywords:
Medium and long chain fat emulsion injection(C8～24Ve);
Emulsion injection;
Particle size distribution;
Laser diffraction method
- From:
Herald of Medicine
2025;44(11):1778-1783
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a laser diffraction method for determining globule size distribution in medium and long chain fat emulsion injection(C8～24Ve).Methods The Mastersizer 3000 laser particle size analyzer and Hydro EV wet sampling device were used,with water as the dispersion medium.Critical parameters,including refractive index,absorption coefficient,obscuration,measurement duration,stirring speed,and sonication parameters,were systematically optimized.Comparative evaluations were conducted across different computational models(Mie vs.Fraunhofer theories)and instrument platforms.Results The optimized optical parameters for particle size distribution analysis of medium and long chain fat emulsion injection(C8～24Ve)were determined as follows:absorption rate 0.001,refractive index 1.52,measurement duration 10 s,stirring speed 1 200 r·min-1,with no sonication applied.Under these conditions,the method demonstrated satisfactory precision,repeatability,and robustness.Conclusions This method demonstrates operational simplicity,high accuracy,and excellent reproducibility,making it suitable for particle size distribution analysis of medium and long chain fat emulsion injection(C8～24Ve).The established protocol provides a valuable reference for the development of particle size determination methods for emulsion formulations.
