Effects of hyperbaric oxygen therapy at different time windows after optic nerve injury in rats by F-VEP
10.3760/cma.j.issn.1009-6906.2019.02.008
- VernacularTitle:F-VEP评估大鼠视神经损伤后不同时间窗高压氧治疗效果
- Author:
Bing LONG
1
;
Liqiong ZHOU
;
Dan YAN
;
Shengping LUO
;
Xu ZHA
;
Xia CAO
;
Zhang LI
;
Xiaoying SUN
;
Ji LI
;
Yuanping ZHANG
Author Information
1. 昆明,昆明医科大学第二附属医院高压氧科
- Publication Type:Journal Article
- Keywords:
Optic nerve injury;
Hyperbaric oxygen;
Different time points;
Visual evoked potential
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2019;26(2):108-111,115
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe flash visual evoked potential (F-VEP)after hyperbaric oxygen (HBO) at different time points after traumatic optic nerve injury (TON) in adult rats,so as to explore the optimal time window for HBO therapy.Methods A total of 40 Sprague-Dawley rats were used to establish an optic nerve contusion model,with the left eye as the optic nerve injury eye and the right eye as the sham surgery control eye.In accordance with different HBO intervention time points of the optic nerve contusion model at 1,3,7 and 14 days,and the animals were divided into 5 groups,i.e.the experimental group 1,group 2,group 3,group 4 and the control group,each consisting of 8 rats.In the course of treatment,F-VEP visual changes in the 5 groups were detected at day 3,day 10 and day 20,and therapeutic effects were evaluated.Results When the detected VEP data of the optic nerve-clamped eye in all the control rats were compared with those of the sham surgical eyes,latent time was prolonged and vibration amplitude was obviously decreased,and statistical significance could be found,when comparisons were made between them (P ＜ 0.05).Compared with the F-VEP data of the control group,latent time of the optic nerve-clamped eye in the experimental group 1 at various time points was considerably shortened,and at day 10 and day 20 after treatment,vibration amplitude was significantly increased,all with statistical significance (P ＜ 0.05).However,latent time of the optic nerve-clamped eye in the experimental group 2 at day 10 and day 20 after treatment was obviously shortened,also with statistical significance (P ＜ 0.05),but no statistical significance could be noted in vibration amplitude,when comparisons were made between them (P ＞ 0.05).When compared with the F-VEP data of the control group,no statistical significance could be found in the changes of latent time and vibration amplitude for the experimental group 3 and group 4 (P ＞ 0.05).Conclusion Early HBO treatment could effectively improve F-VEP of the rats with traumatic optic nerve contusion,and after sustainment of optic nerve injury,the early the HBO intervention,the better therapeutic effects would be achieved.
