Alleviative effects of hyperbaric oxygen on the symptoms of stroke through regulation of NF-κB signal pathway in rats and the mechanism involved
10.3760/cma.j.issn.1009-6906.2019.02.012
- VernacularTitle:高压氧通过调节NF-κB信号通路缓解大鼠脑卒中症状的作用机制
- Author:
Xingguo MU
1
;
Ge HUANG
1
;
Yan CHEN
1
;
Xiaofei XU
1
Author Information
1. 吉林大学第二医院放射科, 长春,130041
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
NF-κB signal pathway;
Inflammatory factor;
Oxidative stress;
Cell apoptosis
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2019;26(2):125-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of hyperbaric oxygen (HBO) on the symptoms of stroke through regulation of nuclear transcription factor-kB (NF-kB) signal pathway in rats and the mechanism involved.Methods Forty SD rats were used in the study and were randomly divided into the sham group,the model group,the model + HBO group and the model + HBO + NF-κBover-expression group.Middle cerebral artery occlusion (MCAO) method was used to develop ischemic stroke rat model.The effects of HBO/NF-κB on cerebral infarction,nerve function,oxidative stress,inflammatory response and brain cell apoptosis were evaluated in the study.Results HBO could significantly reduce the size of brain infarction induced by MCAO (P ＜ 0.05),improve the scores of nerve function (P ＜ 0.05) and enhance the levels of inflammatory response and oxidative stress (P＜0.05 or P ＜0.01) and in the meantime inhibit brain cell apoptosis.However,the over-expression of NF-κB could obviously weaken the therapeutic effects of HBO,and statistical significance could be noticed when comparisons were made between the groups (P ＜ 0.05).Conclusion HBO could alleviate symptoms of cerebral stroke through inhibition of NF-κB activation and improve nerve function,which might be associated with the inhibition of oxidative stress,inflammatory response and brain cell apoptosis.
