Research on the Synergistic Effect and Mechanism of Polysaccharides and Small Molecules in Taohong Siwu Decoction
10.11969/j.issn.1673-548X.2025.09.010
- VernacularTitle:桃红四物汤多糖与小分子的协同作用及机制探究
- Author:
Qingqing XIE
1
;
Xueting ZHANG
;
Xiaoqun WANG
Author Information
1. 230012 合肥,安徽中医药大学药学院
- Publication Type:Journal Article
- Keywords:
Polysaccharide;
Small molecules;
Metabolomics;
Intestinal flora
- From:
Journal of Medical Research
2025;54(9):49-55,35
- CountryChina
- Language:Chinese
-
Abstract:
Objective The effect of total polysaccharides on the absorption of small molecule components in Taohong Siwu Decoction was studied.Methods A rat middle cerebral artery occlusion/reper-fusion(MCAO/R)model was established by using thread occlu-sion method.Intragastric administration of THSWD.THSWD small molecule(THSWDX)and THSWDPX(THSWD polysaccharide plus small molecule)was performed once a day for7days each.Brain,serum,stool and colon were collected after the last dose.TTC staining was used to detect the infarct size,HE staining was used to detect the pathology of colon.16S rRNA high-throughput sequencing was used to detect the changes of intestinal flora in rats.Serum metabolite differences were detected by fully targeted metabolomics.Results In the polysaccharide group,the cerebral infarction size was significantly reduced,the colonic crypt structure was intact,goblet cells were abundant,and the epithelial structure was intact.16S rRNA high-throughput sequencing results showed that compared with the model group,the abundance of Bacteroidetes could be decreased in each administration group,especially in THSWD,and the abundance of Pro-teobacteria,Firmicutes and verrucobacteria could be increased at the same time.The amino acid content in each administration group in-creased while the organic acid content decreased,The abundance of Bacteroidetes in the small molecule group administered alone was still high.and the short-chain fatty acid components such as butyric acid and glutaric acid were missing.and there were differences in trypto-phan metabolism and pyrimidine metabolism pathway.Conclusion Polysaccharides and small molecules have synergistic effects.and their main targets are intestinal flora,which are embodied in the following two aspects:①Polysaccharide can improve the structure of the flora,and promote better drug absorption;②Changes in the intestinal floraffect the changes of metabolites.and different metabolites partic-ipate in body metabolism through different ways to accelerate the recovery of the lesion site.
