Effects of hyperbaric oxygenation combined with ω-3 polyunsaturated fatty acid on TLR4/NF-κB signal pathway in the rats with acute lung injury
10.3760/cma.j.issn.1009-6906.2019.04.001
- VernacularTitle:高压氧联合ω-3多不饱和脂肪酸对急性肺损伤大鼠TLR4/NF-κB信号通路的影响
- Author:
Ling LU
1
;
Lin BAI
1
;
Yuhuai LAN
1
;
Meng WU
1
;
Peiran CONG
1
;
Xin HAO
1
;
Lin ZHANG
1
Author Information
1. 黑龙江省医院重症医学科,哈尔滨,150036
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
ω-3 polyunsaturated fatty acid;
Acute lung injury;
TLR4/NF-κB
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2019;26(4):303-306
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of hyperbaric oxygen (HBO) combined with ω-3 polyunsaturated fatty acid (PUFA ω-3) on lipopolysaccharide-induced acute lung injury in the rats and its related mechanism.Methods Forty-eight SD rats were randomly divided into the control group,the model group,the experimental group and the combined group,each consisting of 12 rats.With exception of the rats in the control group,acute lung injury (ALI) model was established by LPS tracheal instillation in the rats of the model and experimental groups.The experimental group received PUFA ω-3 (150 mg/kg) by gavage,the combined group was treated with HBO on the basis of PUFA omega-3,and equal volume of distilled water was given to the control and model groups.The course of treatment lasted for 14 days.After last medication,pulmonary tissues of the rats were collected and wet/dry ratio was calculated,and the inflammatory factors,TNF-α and IL-1β in the lung tissue homogenate were detected by ELISA test kit.HE staining was used to observe the pathological damage of the lung tissue,and the expression levels of TLR4 and NF-κB proteins in the lung tissue were detected by Western-blotting.Results Compared with the control group,pulmonary edema in the model group could clearly be seen,and the wet/dry ratio of the lung tissue increased markedly (5.57 ± 0.57 vs.4.16 ±0.19) (P ＜0.05).Compared with the model group,the wet/dry ratio of the lung tissue in the experimental and the combined groups decreased (4.78 ±0.25,4.42 ±0.18 vs.5.57 ±0.37) (P ＜0.01 or P ＜ 0.05),and the wet/dry ratio of the combined group was obviously lower than that of the experimental group (4.42 ±0.18 vs.4.78 ±0.25) (P ＜0.05).As compared with the control group,the inflammatory factor levels of TNF-α and IL-1β in the model group all significantly increased(P ＜ 0.01 or P ＜ 0.05).Following treatment with simple PUFA ω-3 or coupled with HBO,the inflammatory factor levels of TNF-α and IL-1β in lung tissues of the experimental and the combined groups all decreased markedly,as compared with those of the model group (P ＜ 0.05),with those of the combined group decreased more significantly (P ＜ 0.05).As compared with those of the control group,the expression levels of TLR4 and NF-κB proteins in the model group were significantly up-regulated (P ＜ 0.01).Compared with the model group,the expression levels of TLR4 and NF-κB proteins in the experimental and the combined groups all decreased(P ＜0.01 or P ＜0.05),with the down-regualtion of TLR4 in the experimental group displaying more markedly (P ＜ 0.01).Compared with the experimental group,the down-regulation of NF-κB protein in the combined group was more significant (P ＜ 0.05).Conclusion HBO could obviously enhance the protective effect of PUFA ω-3 on the lung tissue of the rats with ALI and reduce the level of inflammatory factors,which might be associated with the regulation of TLR4/NF-κB signal pathway.
