Enhanced effect of miR-199a-3p on glioma U251 via regulation of PAK4
10.3760/cma.j.issn.1009-6906.2019.04.001
- VernacularTitle:miR-199a-3p通过调控PAK4对胶质瘤U251细胞放疗敏感性的增强作用
- Author:
Chunshan YAO
1
;
Xingguo MU
Author Information
1. 吉林大学第二医院脑外科,长春,130041
- Publication Type:Journal Article
- Keywords:
Radiosensitivity;
Glioma;
miR-199a-3p;
Protein activated kinase 4;
PAK4
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2019;26(4):332-336
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the enhanced effect of miR-199a-3p on radiotherapy sensitivity of glioma U251 by the targeting p21 protein activated kinase 4 (PAK4).Methods Human malignant glioma U251 was chosen for study.Following culture and radiation of the harvested cells,RT-PCR was used to detect the expression level of miR-199a-3p in the glioma tissues and cells of U251,CCK-8,cloning formation assay and flow cytometry were used to detect the effects of miR-199a-3p/PAK4 on the proliferation,cloning formation and apoptosis of glioma U251 cells when treated with irradiation.Results As compared with the U251 cells without radiation,the expression level of miR-199a-3p in the irradiated glioma tissues was lower(P ＜ 0.05).The overexpression of miR-199a-3p could reduce the growth and clonal formation of U251 cells,and promoted cell apoptosis,when treated with 4 Gy-irradiation.Statistical significance could be noted,when comparisons were made between them(P ＜ 0.05 or P ＜ 0.01) MiR-199a-3p could inhibit the expression of PAK4 by direct binding to the 3'UTR of PAK4.Moreover,the overexpression of PAK4 could significantly weaken the roles of miR-199a-3p in the inhibition of U251 cell activity.Conclusion This study has demonstrated that miR-199a-3p could enhance the radiosensitivity of glioma by inhibiting the expression of PAK4.
